Unravelling the potential of seaweeds from the Black Sea coast of Romania as bioactive compounds sources. Part I: Cystoseira barbata (Stackhouse) C. Agardh
| Autor: | Alexandra Jitareanu, Laura Bucur, Anca Miron, Alexandru Vasincu, Christiana M. Neophytou, Evelyn Wolfram, Ana Clara Aprotosoaie, Simon Vlad Luca, Oana Cioanca, Andreas I. Constantinou, Monica Hancianu, Adriana Trifan, Bogdan I. Cioroiu, Sebastian E. W. Opitz, Daciana Sava |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
Phlorotannins medicine.medical_treatment Phloroglucinol Brown algae Apoptosis Toxicology Antioxidants Inhibitory Concentration 50 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Nutraceutical Algae Botany medicine Humans Cell Proliferation 030304 developmental biology Biological Products 0303 health sciences biology Romania 572: Biochemie Chemistry Eckol 04 agricultural and veterinary sciences General Medicine Seaweed biology.organism_classification 040401 food science A549 Cells MCF-7 Cells Sargassaceae DNA fragmentation Female Reactive Oxygen Species HT29 Cells Tannins Anti-proliferative activity Food Science |
| Zdroj: | Food and Chemical Toxicology. 134:110820 |
| ISSN: | 0278-6915 |
| Popis: | The Romanian coastlines of the Black Sea have abundant seaweed resources, but little effort has been done to investigate their biological potential. The aim of the present study was to assess the in vitro antioxidant and anti-proliferative effects of Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae), a brown alga inhabiting the Black Sea coast of Romania. The 70% acetone, methanol and water extracts of C. barbata were evaluated for their total phenolic content, antioxidant activity and anti-proliferative potential against human tumor cell lines (pulmonary A549, colon HT-29, mammary MCF-7) and the non-tumor mammary epithelial MCF-10A cell line. C. barbata 70% acetone extract (CBAE) displayed the highest antioxidant and cytotoxic activities. The mechanism of CBAE anti-proliferative activity involved initially increased intracellular ROS accumulation, followed by increased DNA content in the subG1 phase and DNA fragmentation leading to excessive apoptosis. Thus, our study provides a theoretical basis for the use of CBAE as a tumor preventive agent. Furthermore, UHPLC-DAD-QTOF-MS analysis of CBAE tentatively identified 18 phlorotannins as fucophlorethol and eckol derivatives, containing three up to seven phloroglucinol units. In conclusion, C. barbata represents a valuable source for the development of macroalgal-based products with putative use as nutraceuticals and pharmaceuticals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect