Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells
| Autor: | Angela Nebbioso, Mariarosaria Conte, Rosario Esposito, Marco Micciarelli, Carlo Altucci, Rosaria Benedetti, Vincenzo Carafa, Lucia Altucci, Raffaele Velotta |
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| Přispěvatelé: | Altucci, C, Nebbioso, Angela, Benedetti, R, Esposito, R, Carafa, V, Conte, M, Micciarelli, M, Altucci, Lucia, Velotta, R., Altucci, Carlo, A., Nebbioso, R., Benedetti, Esposito, Rosario, V., Carafa, M., Conte, Micciarelli, Marco, L., Altucci, Velotta, Raffaele |
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Physics and Astronomy (miscellaneous) ChIP RNA POLYMERASE-II Laser 01 natural sciences law.invention femtosecond probing of biological molecules 03 medical and health sciences bioploymer law 0103 physical sciences Phenomenological model medicine IRRADIATION crosslinking Irradiation 010306 general physics Absorption (electromagnetic radiation) Instrumentation Cell damage 030304 developmental biology 0303 health sciences business.industry Nonlinear optics DNA medicine.disease nonlinear optic Femtosecond Nucleic acid Biophysics Optoelectronics business |
| Zdroj: | Laser physics letters 9 (2012): 234–239. doi:10.1002/lapl.201110122 info:cnr-pdr/source/autori:Altucci C., Nebbioso A., Benedetti R., Esposito R., Carafa V., Conte M., Micciarelli M., Altucci L., Velotta R./titolo:Nonlinear protein-nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells/doi:10.1002%2Flapl.201110122/rivista:Laser physics letters (Print)/anno:2012/pagina_da:234/pagina_a:239/intervallo_pagine:234–239/volume:9 Laser Physics Letters; Vol 9 Laser Physics Letters |
| ISSN: | 1612-2011 |
| DOI: | 10.1002/lapl.201110122 |
| Popis: | ""We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser. pulses. DNA-protein crosslinking is obtained with two different. techniques: conventional chemical or laser-induced. Our results. evidence a nonlinear response of the cells to the laser irradiation. in the investigated intensity range. The laser induced crosslink. efficiency can reach a value approximately twice higher than. that obtained with ordinary chemical methods. The experimental. results are very well reproduced by a simple phenomenological. model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse.. "" |
| Databáze: | OpenAIRE |
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