CD28 negative T cells are enriched in granulomatous lesions of the respiratory tract in Wegener's granulomatosis
| Autor: | Elena Csernok, A. Schnabel, A Mueller, U Seitzer, Wolfgang L. Gross, P Lamprecht, F Moosig |
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| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Sarcoidosis T-Lymphocytes T cell chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Statistics Nonparametric CD28 Antigens Biopsy medicine Humans Aged Immunity Cellular medicine.diagnostic_test business.industry Granulomatosis with Polyangiitis CD28 hemic and immune systems Original Articles T lymphocyte Middle Aged medicine.disease Immunohistochemistry medicine.anatomical_structure Bronchoalveolar lavage Granuloma business Bronchoalveolar Lavage Fluid CD8 Respiratory tract |
| Zdroj: | Thorax. 56:751-757 |
| ISSN: | 0040-6376 |
| DOI: | 10.1136/thorax.56.10.751 |
| Popis: | BACKGROUND—Lack of CD28 expression on peripheral blood CD4+ and CD8+ T cells has been reported in patients with Wegener's granulomatosis (WG), suggesting a pathogenetic role of CD28- T cells in WG. METHODS—Ten patients with WG and six with sarcoidosis (disease control) were analysed. Fluorescence activated cell sorter (FACS) analysis was used to detect CD28 expression on T cells from peripheral venous blood and from bronchoalveolar lavage (BAL) fluid. T cells in biopsy specimens from granulomatous lesions of the upper respiratory tract were analysed for CD28 expression by double immunofluorescence staining. RESULTS—A significantly higher fraction of CD28- T cells was found in the CD4+ and CD8+ T cell compartment in BAL fluid (65.6 (5.4)% and 76.3 (4.1)%, respectively) than in blood (13.4 (6.2)% and 42.9 (6.2)%; p |
| Databáze: | OpenAIRE |
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