Bulge loops used to measure the helical twist of RNA in solution
| Autor: | Roderick S. Tang, David E. Draper |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Gel electrophoresis
Models Molecular Quantitative Biology::Biomolecules Base Sequence Chemistry Base pair Molecular Sequence Data Analytical chemistry RNA Astrophysics::Cosmology and Extragalactic Astrophysics Quantitative Biology::Genomics Biochemistry Turn (biochemistry) Crystallography Bulge Helix Nucleic Acid Conformation Electrophoresis Polyacrylamide Gel Twist Polyacrylamide gel electrophoresis Astrophysics::Galaxy Astrophysics RNA Double-Stranded |
| Zdroj: | Biochemistry. 29(22) |
| ISSN: | 0006-2960 |
| Popis: | Bulge loops are commonly found in helical segments of cellular RNAs. When incorporated into long double-stranded RNAs, they may introduce points of flexibility or permanent bend that can be detected by the altered electrophoretic gel mobility of the RNA. We find that a single An or Un bulge loop near the middle of a long RNA helix significantly retards the RNA during polyacrylamide gel electrophoresis if n greater than or equal to 2. The mobility of an RNA containing two A2 bulges various periodically with the number of base pairs between the bulges. We interpret this to mean that A2 bulges varies periodically with the number of base pairs between the bulges. We interpret this to mean that Z2 bulges form torsionally stiff bends in the helix; the gel mobility reaches a minimum when the total helical twist between the bulges rotates the arms of the molecule into a cis conformation. The gel mobilities are proportional to the predicted end-to-end distance of the RNA if the average RNA helical repeat is 11.8 +/- 0.2 bp/turn and there is no helical twist (3 +/- 9 degrees) associated with the bulge (data obtained in 0.15 M Na+). Other sizes and sequences of bulges have very different effects on RNA helix conformation and flexibility. U2 bulges bend the helix to a much smaller degree than A2 bulges, while longer A or U bulge sequences probably allow bends of 90 degrees or more; all of these may be fairly flexible joints.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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