Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations

Autor: Ivan da Rocha Pitta, Rafaela Silva Guimarães Gonçalves, Sayonara Maria Calado Gonçalves, Maria Clara Pinheiro Duarte Sampaio, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Claudia Diniz Lopes Marques, Anderson Rodrigues de Almeida, Kamila de Melo Vilar, Michelly Cristiny Pereira, Andréa Tavares Dantas, Angela Luzia Branco Pinto Duarte
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Adult
Male
Article Subject
medicine.medical_treatment
Biopsy
Clinical Biochemistry
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
Peripheral blood mononuclear cell
Scleroderma
Transforming Growth Factor beta1
03 medical and health sciences
Young Adult
0302 clinical medicine
Genetics
medicine
Humans
RNA
Messenger
skin and connective tissue diseases
Molecular Biology
Aged
Skin
030203 arthritis & rheumatology
lcsh:R5-920
Scleroderma
Systemic
integumentary system
business.industry
Biochemistry (medical)
CD28
General Medicine
Middle Aged
Serum samples
medicine.disease
Prognosis
030104 developmental biology
Cytokine
Case-Control Studies
Immunology
Leukocytes
Mononuclear
Biomarker (medicine)
Cell culture supernatant
Female
business
lcsh:Medicine (General)
Biomarkers
Transforming growth factor
Research Article
Follow-Up Studies
Zdroj: Disease Markers
Disease Markers, Vol 2016 (2016)
ISSN: 0278-0240
DOI: 10.1155/2016/6064830
Popis: Objective. To determine active TGF-β1 (aTGF-β1) levels in serum, skin, and peripheral blood mononuclear cell (PBMC) culture supernatants and to understand their associations with clinical parameters in systemic sclerosis (SSc) patients.Methods. We evaluated serum samples from 56 SSc patients and 24 healthy controls (HC). In 20 SSc patients, we quantified spontaneous or anti-CD3/CD28 stimulated production of aTGF-β1 by PBMC. The aTGF-β1 levels were measured by ELISA. Skin biopsies were obtained from 13 SSc patients and six HC, and TGFB1 expression was analyzed by RT-PCR.Results. TGF-β1 serum levels were significantly higher in SSc patients than in HC (p< 0.0001). Patients with increased TGF-β1 serum levels were more likely to have diffuse subset (p= 0.02), digital ulcers (p= 0.02), lung fibrosis (p< 0.0001), positive antitopoisomerase I (p= 0.03), and higher modified Rodnan score (p= 0.046). Most of our culture supernatant samples had undetectable levels of TGF-β1. No significant difference in TGFB1 expression was observed in the SSc skin compared with HC skin.Conclusion. Raised active TGF-β1 serum levels and their association with clinical manifestations in scleroderma patients suggest that this cytokine could be a marker of fibrotic and vascular involvement in SSc.
Databáze: OpenAIRE
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