Viable offspring obtained from Prm1-deficient sperm in mice
| Autor: | Naoki Takeda, Shin Ichi Abe, Shin Aizawa, Zhenghua Li, Kazuya Yoshinaga, Kazufumi Takamune, Ken Ichi Yamamura, Kenryo Furushima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Blastomeres Offspring medicine.medical_treatment Fertilization in Vitro Article Andrology Histones 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine medicine Animals Protamines Membrane Potential Mitochondrial 030219 obstetrics & reproductive medicine Multidisciplinary In vitro fertilisation biology Spermatid urogenital system DNA Embryo Transfer Sperm Protamine Molecular biology Spermatids Spermatozoa Chromatin 030104 developmental biology Histone medicine.anatomical_structure chemistry biology.protein Oocytes Female |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Protamines are expressed in the spermatid nucleus and allow denser packaging of DNA compared with histones. Disruption of the coding sequence of one allele of either protamine 1 (Prm1) or Prm2 results in failure to produce offspring, although sperm with disrupted Prm1 or Prm2 alleles are produced. Here, we produced Prm1-deficient female chimeric mice carrying Prm1-deficient oocytes. These mice successfully produced Prm1+/− male mice. Healthy Prm1+/− offspring were then produced by transferring blastocysts obtained via in vitro fertilization using zona-free oocytes and sperm from Prm1+/− mice. This result suggests that sperm lacking Prm1 can generate offspring despite being abnormally shaped and having destabilised DNA, decondensed chromatin and a reduction in mitochondrial membrane potential. Nevertheless, these mice showed little derangement of expression profiles. |
| Databáze: | OpenAIRE |
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