Building a DNA barcode library of Alaska’s non-marine arthropods
| Autor: | Sarah Meierotto, Matthew L. Bowser, John M. Morton, Casey Bickford, Kyndall B.P. Hildebrandt, Derek S. Sikes |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Canada Insecta 010607 zoology Biodiversity Biology Barcode 010603 evolutionary biology 01 natural sciences DNA barcoding law.invention Species Specificity law Genetics Animals DNA Barcoding Taxonomic Arthropods Molecular Biology Phylogeny Gene Library Trophic level Ecology Geography Models Genetic Temperature Genetic Variation DNA Sequence Analysis DNA General Medicine biology.organism_classification Dna barcodes Wildlife refuge Arthropod Alaska Biotechnology |
| Zdroj: | Genome. 60:248-259 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2015-0203 |
| Popis: | Climate change may result in ecological futures with novel species assemblages, trophic mismatch, and mass extinction. Alaska has a limited taxonomic workforce to address these changes. We are building a DNA barcode library to facilitate a metabarcoding approach to monitoring non-marine arthropods. Working with the Canadian Centre for DNA Barcoding, we obtained DNA barcodes from recently collected and authoritatively identified specimens in the University of Alaska Museum (UAM) Insect Collection and the Kenai National Wildlife Refuge collection. We submitted tissues from 4776 specimens, of which 81% yielded DNA barcodes representing 1662 species and 1788 Barcode Index Numbers (BINs), of primarily terrestrial, large-bodied arthropods. This represents 84% of the species available for DNA barcoding in the UAM Insect Collection. There are now 4020 Alaskan arthropod species represented by DNA barcodes, after including all records in Barcode of Life Data Systems (BOLD) of species that occur in Alaska — i.e., 48.5% of the 8277 Alaskan, non-marine-arthropod, named species have associated DNA barcodes. An assessment of the identification power of the library in its current state yielded fewer species-level identifications than expected, but the results were not discouraging. We believe we are the first to deliberately begin development of a DNA barcode library of the entire arthropod fauna for a North American state or province. Although far from complete, this library will become increasingly valuable as more species are added and costs to obtain DNA sequences fall. |
| Databáze: | OpenAIRE |
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