Genetic dissection of the final exocytosis steps in Paramecium tetraurelia cells: cytochemical determination of Ca2+-ATPase activity over performed exocytosis sites

Autor:	H, Plattner, K, Reichel, H, Matt, J, Beisson, M, Lefort-Tran, M, Pouphile
Rok vydání:	1980
Předmět:	Organoids Cytoplasm Microscopy Electron Paramecium Cell Membrane Mutation Animals Calcium-Transporting ATPases Cell Biology Exocytosis Electron Probe Microanalysis
Zdroj:	Journal of Cell Science. 46:17-40
ISSN:	1477-9137 0021-9533
DOI:	10.1242/jcs.46.1.17
Popis:	In different Paramecium tetraurelia strains the occurrence of a Ca2+-ATPase (or p-nitro-phenylphosphatase) activity at the preformed attachment and exocytosis sites of the secretory vesicles (trichocysts) was analysed by electron-microscopic cytochemistry and X-ray microanalysis. In conjunction with freeze-fracture studies it was found that only those strains, which contain rosette particles, display this Ca2+-ATPase activity (7S, K 40I, nd 9 (18 degrees C), while other strains (nd 6, nd 9 (27 degrees C), tam 38) are devoid of both these characteristics. The presence (absence) of rosette particles and of Ca2+-ATPase activity at the preformed exocytosis sites is correlated with the capability (incapability) of performing exocytosis in these strains. We discuss several possible interpretations of this structure-function correlation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c163930b51e4608bc000b0a865153afa https://doi.org/10.1242/jcs.46.1.17 Zobrazit plný text záznamu