Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family
| Autor: | Teri L. Aldrich, R K Rothmel, J. E. Houghton, L. N. Ornston, Wayne Coco, Ananda M. Chakrabarty |
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| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Operon Muconate cycloisomerase Molecular Sequence Data Restriction Mapping Biology Benzoates Microbiology Bacterial Proteins Pseudomonas Sequence Homology Nucleic Acid Genes Regulator Escherichia coli Inducer Amino Acid Sequence Cloning Molecular Intramolecular Lyases Isomerases Molecular Biology Peptide sequence Regulator gene Genetics Base Sequence Nucleic acid sequence Promoter Carbon-Carbon Double Bond Isomerases biology.organism_classification Pseudomonas putida RNA Bacterial Biochemistry Genes Bacterial Protein Biosynthesis Plasmids Research Article |
| Zdroj: | Journal of Bacteriology. 172:922-931 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Pseudomonas putida utilizes the catBC operon for growth on benzoate as a sole carbon source. This operon is positively regulated by the CatR protein, which is encoded from a gene divergently oriented from the catBC operon. The catR gene encodes a 32.2-kilodalton polypeptide that binds to the catBC promoter region in the presence or absence of the inducer cis-cis-muconate, as shown by gel retardation studies. However, the inducer is required for transcriptional activation of the catBC operon. The catR promoter has been localized to a 385-base-pair fragment by using the broad-host-range promoter-probe vector pKT240. This fragment also contains the catBC promoter whose -35 site is separated by only 36 nucleotides from the predicted CatR translational start. Dot blot analysis suggests that CatR binding to this dual promoter-control region, in addition to inducing the catBC operon, may also regulate its own expression. Data from a computer homology search using the predicted amino acid sequence of CatR, deduced from the DNA sequence, showed CatR to be a member of a large class of procaryotic regulatory proteins designated the LysR family. Striking homology was seen between CatR and a putative regulatory protein, TfdS. |
| Databáze: | OpenAIRE |
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