Vaccination Against Schistosomiasis in Mice With Killed Schistosomula without Adjuvant
| Autor: | H. F. Hsü, S. Y. Li Hsü, William T. Clarke, Kent W. Svestka |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Vaccines
Cellular immunity medicine.medical_treatment Vaccination Schistosomiasis Biology medicine.disease Virology Schistosomiasis mansoni General Biochemistry Genetics and Molecular Biology Mice Antigen Delayed hypersensitivity parasitic diseases Immunology medicine Animals Female Hypersensitivity Delayed Immune reaction Adjuvant Skin Tests Killed Vaccine |
| Zdroj: | Experimental Biology and Medicine. 181:454-458 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-181-3-rc2 |
| Popis: | Attempts to develop a killed vaccine against schistosomiasis have generally resulted in failure. There are two recent reports, but unfortunately, harsh adjuvants were used in conjunction with the antigenic materials. In our laboratory, a killed vaccine was developed by freezing (-196 degrees C) and thawing the schistosomula of S. mansoni. The use of such a preparation without adjuvant was effective in vaccinating mice. A worm reduction of 36.4-41.1% was achieved by one vaccinating injection, a 60.2% worm reduction by 3 injections, and a 63.7-66.0% reduction by 5 injections. The sequence of the development and the expression of the immune reactions were similar to those previously found in hosts immunized with highly X-irradiated schistosome organisms. Delayed hypersensitivity was demonstrated in histological sections of the skin in the challenged mice after one vaccination, showing that an adjuvant was not necessary to initiate the induction of cellular immunity. |
| Databáze: | OpenAIRE |
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