A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactions
| Autor: | Simone P. Niclou, Rolf Bjerkvig, Per Øyvind Enger, Anja Torsvik, Hans Petter Eikesdal, Aurélie Poli, Claude Danzeisen, Helge Wiig, Nicolaas H. C. Brons, Agnete Svendsen, Jorge A. Terzis |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cell signaling
Green Fluorescent Proteins Cell Communication Mice SCID Biology Biochemistry Green fluorescent protein law.invention Research Communications Mice Confocal microscopy law Mice Inbred NOD glioma Neoplasms Genetics tumor microenvironment Animals Humans Vasculogenic mimicry vascular mimicry xenograft Molecular Biology Tumor microenvironment Cell fusion cell fusion Colocalization Cell sorting Molecular biology Cell biology Microscopy Fluorescence Multiphoton in vivo imaging Neoplasm Transplantation Biotechnology |
| Zdroj: | The FASEB Journal |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | A NOD/Scid mouse expressing enhanced green fluorescent protein (eGFP) is described, in which human and mouse tumors marked with red fluorescent protein can be established in vivo, both at subcutaneous and orthotopic locations. Using light microscopy as well as multiphoton confocal microscopy techniques, we visualized in detail the intricate colocalization of tumor and host cells in situ. Moreover, using fluorescence-activated cell sorting (FACS), we were able to completely separate the host cells from the tumor cells, thus providing a system for detailed cellular and molecular analysis of tumor-host cell interactions. The fact that tumor and host cells can be reliably identified also allowed us to detect double-positive cells, possibly arising from cell fusion events or horizontal gene transfer. Similarly, the model can be applied for the detection of circulating metastatic cells and for detailed studies on the vascular compartments within tumors, including vasculogenic mimicry. Thus, the model described should provide significant insight into how tumor cells communicate with their microenvironment.—Niclou, S. P., Danzeisen, C., Eikesdal, H. P., Wiig, H., Brons, N. H. C., Poli, A. M. F., Svendsen, A., Torsvik, A., Enger, P. O., Terzis, J. A., Bjerkvig, R. A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactions. |
| Databáze: | OpenAIRE |
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