Analysis of HER-2/neu amplification in endometrial carcinoma by chromogenic in situ hybridization. Correlation with fluorescence in situ hybridization, HER-2/neu, p53 and Ki-67 protein expression, and outcome
| Autor: | Gloria Peiró, P Hillemanns, Doris Mayr, Joachim Diebold, Udo Löhrs |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Receptor ErbB-2 Chromogenic in situ hybridization In situ hybridization Biology Pathology and Forensic Medicine Carcinoma medicine Humans CISH In Situ Hybridization In Situ Hybridization Fluorescence Survival analysis Aged Aged 80 and over medicine.diagnostic_test Gene Amplification Genes erbB-2 Middle Aged medicine.disease Immunohistochemistry Survival Analysis Molecular biology Endometrial Neoplasms Ki-67 Antigen Ki-67 biology.protein Female Tumor Suppressor Protein p53 Fluorescence in situ hybridization |
| Zdroj: | Modern Pathology. 17:277-287 |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.3800006 |
| Popis: | Fluorescence in situ hybridization (FISH) is the most widely used technique to detect HER-2/neu gene amplification; however, it is only available in some institutions. In contrast, chromogenic in situ hybridization (CISH) can be evaluated by routine light microscopy. In endometrial carcinoma there are few data concerning HER-2/neu status and prognosis. Therefore, we determined HER-2/neu gene status by CISH using a digoxigenin-labelled probe on 60 formalin-fixed paraffin-embedded endometrial carcinomas. The data were compared with the immunohistochemistry of HER-2/neu (A0485, TAB250), p53, Ki-67, clinicopathological factors, and survival. By conventional light microscopy, HER-2/neu amplification (>/=6 copies >50% cancer cells) was detected in 14% (8/59) tumours, HER-2/neu overexpression (>10% cells moderate/strong complete membrane staining) in 22% (13/60) for A0485, and 18% (11/60) for TAB250, p53 (>10% +cells) in 61% (36/59), and Ki-67 (>50% +cells) in 50% (30/60). Discordant cases for CISH and immunohistochemistry, as well as all (2+) were further analysed by FISH (Vysis). Among 10 cases (2+) and not amplified by CISH, two showed low-level amplification by FISH. Significant correlation was found between amplification and protein overexpression (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|