Photofrin, but not benzoporphyrin derivative, stimulates hematopoiesis in the mouse
| Autor: | David W. C. Hunt, Robert A. Sorrenti, Claire Smits, Julia G. Levy |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Male Porphyrins Light Ratón medicine.medical_treatment CFU-GM Population Spleen Photodynamic therapy Bone Marrow Cells Cell Count Pharmacology Biology Colony-Forming Units Assay Leukocyte Count Mice In vivo Bone Marrow medicine Concanavalin A Animals education Cells Cultured Immunosuppression Therapy education.field_of_study Hematopoiesis Haematopoiesis medicine.anatomical_structure Mice Inbred DBA Immunology Dihematoporphyrin Ether Bone marrow Fluorouracil |
| Zdroj: | Immunopharmacology. 26(3) |
| ISSN: | 0162-3109 |
| Popis: | This report describes the effects of the porphyrin photosensitizers, Photofrin ® and benzoporphyrin derivative (BPD) on the immunohematopoietic system of normal and immunosuppressed DBA/2 mice in the absence of activating light. Photofrin ® (10 and 25 mg/kg) significantly increased in vitro colony formation by cells of the granulocyte-macrophage lineage in the spleen and bone marrow. Splenic hypercellularity, splenomegaly and elevated levels of blood leukocytes were observed in these mice 7 days following Photofrin ® injection. Evidence that Photofrin ® influenced the lymphohematopoietic compartment was suggested by a significant increase in blood lymphocytes and a population of spleen cells identified by a monoclonal antibody (LR-1) reactive with mouse splenic B lymphocytes. Proliferative reponses of spleen cells from Photofrin ® -treated mice to sub-optimal concentrations of Con A were greater than that observed for controls. However, spleen cell responses to LPS were unaltered by Photofrin ® administration. In contrast, BPD (10 mg/kg) did not alter any of the immunohematopoietic parameters studied. When Photofrin ® was administered to mice treated with the myeloablative agent 5-FU there was a significant acceleration in the recovery of total blood leukocyte and spleen cell numbers, relative to the controls. These studies demonstrate that, in addition to its previously documented activities as a photosensitizer, Photofrin ® can exert stimulatory effects upon murine hematopoiesis. |
| Databáze: | OpenAIRE |
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