Postoperative recurrence of epithelial ovarian cancer patients and chemoresistance related protein analyses
| Autor: | Kaiyun Qin, Zhengmao Zhang, Botao Yang, Bao-en Shan, Fenghua Zhang, Lianmei Zhao, Xiaojing Zhang, Chen Zhao, Wenzhe Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Proteomics Proteome Down-Regulation Complement factor I Carcinoma Ovarian Epithelial Immunoglobulin light chain lcsh:Gynecology and obstetrics 03 medical and health sciences 0302 clinical medicine Biomarkers Tumor Humans Medicine lcsh:RG1-991 Aged Ovarian Neoplasms Gel electrophoresis Clusterin biology Differentially expressed proteins business.industry Research Haptoglobin Obstetrics and Gynecology Middle Aged Epithelial ovarian cancer Blood proteins Up-Regulation Transthyretin Two dimensional gelelectrophoresis 030104 developmental biology Oncology Drug Resistance Neoplasm Time of flight mass spectrometry 030220 oncology & carcinogenesis biology.protein Cancer research Female Neoplasm Recurrence Local business Chemotherapy resistance |
| Zdroj: | Journal of Ovarian Research, Vol 12, Iss 1, Pp 1-8 (2019) Journal of Ovarian Research |
| ISSN: | 1757-2215 |
| DOI: | 10.1186/s13048-019-0499-z |
| Popis: | Objective To identify the plasma protein biomarkers related to the chemoresistance of postoperative recurrence of epithelial ovarian cancers. Methods Forty plasma samples from patients in chemotherapy-sensitive and chemotherapy-resistant groups (20 for each group) were collected at Gynecology Department in the Fourth Hospital of Hebei Medical University from September 2013 to September 2014. The differentially expressed proteins between two groups were screened with two-dimensional gel electrophoresis (2-DE) and further analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF–MS). Results Thirty-four differentially expressed spots were identified between the two groups. Compared with the chemo-sensitive group, 21 protein spots were up-regulated and 13 were down-regulated in the chemoresistant group, in which 14 differentially expressed proteins were identified by the Mass spectrometry and Mascot search. Among the 14 proteins, complement C4-A, IgJ chain, clusterin, α-1-antitrypsin and carbonic anhydrase 1 were up-regulated, and transthyretin, haptoglobin, β-2-glycoprotein, Ig γ-2 chain C region, Ig γ-1 chain C region, complement factor I light chain, Igκ chain C region, complement C3 and apolipoprotein E were down-regulated in the chemoresistant group when compared with the chemosensitive group. Conclusion The up-regulated proteins including transthyretin, apolipoprotein E and haptoglobin proteins and the down-regulated proteins such as clusterin, carbonic anhydrase 1, alpha-1-antitrypsin were differentially expressed in the plasma between the chemo-sensitive group and the chemoresistant group, which may be potential biomarkers for predicting the chemotresistance of epithelial ovarian cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |