Leveraging copper import by yersiniabactin siderophore system for targeted PET imaging of bacteria
| Autor: | Nitin S. Kamble, Suzanne E. Lapi, Nalinikanth Kotagiri, Robert O’Donnell, Hailey A. Houson, Daniel J. Hassett, Jose R. Blanco, Nabil A. Siddiqui |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Siderophore Siderophores Computational biology Metal Chelator Yersiniabactin Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phenols In vivo Animals Mice Inbred BALB C Infectious disease Bacteria biology Chemistry Bacterial Infections General Medicine Pet imaging biology.organism_classification Respiratory enzyme Molecular Imaging Disease Models Animal Thiazoles 030104 developmental biology Technical Advance Echocardiography Positron-Emission Tomography 030220 oncology & carcinogenesis Diagnostic imaging Female Copper Function (biology) |
| Zdroj: | JCI Insight |
| ISSN: | 2379-3708 |
| Popis: | There is an emerging need for accurate and rapid identification of bacteria in the human body to achieve diverse biomedical objectives. Copper homeostasis is vital for the survival of bacterial species owing to the roles of the metal as a nutrient, respiratory enzyme cofactor, and a toxin. Here, we report the development of a copper-64–labeled bacterial metal chelator, yersiniabactin, to exploit a highly conserved metal acquisition pathway for noninvasive and selective imaging of bacteria. Compared with traditional techniques used to manufacture probes, our strategy simplifies the process considerably by combining the function of metal attachment and cell recognition to the same molecule. We demonstrate, for the first time to our knowledge, how a copper-64 PET probe can be used to identify specific bacterial populations, monitor antibiotic treatment outcomes, and track bacteria in diverse niches in vivo. |
| Databáze: | OpenAIRE |
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