Influence of knockout of At4g20990 gene encoding α-CA4 on photosystem II light-harvesting antenna in plants grown under different light intensities and day lengths
Autor: | E. M. Zhurikova, Tatyana P. Fedorchuk, N. N. Rudenko, L. K. Ignatova, Boris Ivanov, D. V. Vetoshkina |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Photosystem II Light Non-photochemical quenching Mutant Arabidopsis Light-Harvesting Protein Complexes food and beverages Photosystem II Protein Complex Context (language use) Cell Biology Plant Science General Medicine Biology Photosynthesis 01 natural sciences 03 medical and health sciences Light intensity 030104 developmental biology Photoprotection Botany Biophysics Gene knockout 010606 plant biology & botany |
Zdroj: | Protoplasma. 255(1) |
ISSN: | 1615-6102 |
Popis: | Effect of knockout of the At4g20990 gene encoding α-carbonic anhydrase 4 (α-CA4) in Arabidopsis thaliana in plants grown in low light (LL, 80 μmol photons m-2 s-1) or in high light (HL, 400 μmol photons m-2 s-1) under long (LD, 16 h) or short (SD, 8 h) day length was studied. In α-CA4 knockout plants, under all studied conditions, the non-photochemical quenching was lower; the decrease was more pronounced under HL. This pointed to α-CA4 implication in the processes leading to energy dissipation in PSII antenna. In this context the content of major antenna proteins Lhcb1 and Lhcb2 was lower in α-CA4 knockouts than in wild-type (WT) plants under all growth conditions. The expression level of lhcb2 gene was also lower in mutants grown under LD, LL and HL in comparison to WT. At the same time, this level was higher in mutants grown under SD, LL and it was the same under SD, HL. Overall, the data showed that the knockout of the At4g20990 gene affected both the contents of proteins of PSII light-harvesting complex and the expression level of genes encoding these proteins, with peculiarities dependent on day length. These data together with the fact of a decrease of non-photochemical quenching of leaf chlorophyll a fluorescence in α-CA4-mut as compared with that in WT plants implied that α-CA4 participates in acclimation of photosynthetic apparatus to light intensity, possibly playing important role in the photoprotection. The role of this CA can be especially important in plants growing under high illumination conditions. |
Databáze: | OpenAIRE |
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