Cell Membrane Polarity of the Epithelial Cells in the Endolymphatic Sac of the Guinea Pig
| Autor: | J. C. M. J. De Groot, F. W. J. Albers, J. E. Veldman, P. P. G. van Benthem, Egbert H. Huizing |
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| Rok vydání: | 1992 |
| Předmět: |
Guinea Pigs
Hyaluronoglucosaminidase Neuraminidase Epithelium Endolymphatic sac Glycocalyx Cell membrane Polysaccharides medicine Animals Inner ear Cilia Glycoproteins Basement membrane Chemistry Cell Membrane Thorium Epithelial Cells Cochlea Membrane medicine.anatomical_structure Otorhinolaryngology Ferritins Immunology Cytochemistry Biophysics Female Endolymphatic Sac |
| Zdroj: | ORL. 54:241-245 |
| ISSN: | 1423-0275 0301-1569 |
| DOI: | 10.1159/000276306 |
| Popis: | The epithelial cells of the endolymphatic sac (ES) were studied in order to characterize their glycocalyx composition. Colloidal thorium and cationized ferritin were used as electron-dense markers to visualize the glycocalyx as well as the basement membrane. In addition, enzymatic digestions were performed to identify the reactive components of the glycocalyx responsible for colloidal thorium labeling. The results indicate that the glycocalyx of the ES epithelial lining is very rich in sialic acid. Furthermore, a marked polarity in glycocalyx reactivity is present between the apical and basolateral membranes, whereas difference in glycocalyx reactivity between the light and the dark cells are not obvious. We speculate that the specific composition and the apparent polarity of the ES epithelial cell glycocalyx are of importance to the equilibrium of electrolytes in the ES lumen. |
| Databáze: | OpenAIRE |
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