Radiotherapy combined with intratumoral dendritic cell vaccination enhances the therapeutic efficacy of adoptive T-cell transfer
| Autor: | Qiao Li, Kari Wilder-Romans, Janet B. Hoff, Seagal Teitz-Tennenbaum, Mu Li, Alfred E. Chang, Mary A. Davis |
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| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cancer Research Adoptive cell transfer Liver tumor Lung Neoplasms medicine.medical_treatment T cell Immunology Melanoma Experimental Antineoplastic Agents CD8-Positive T-Lymphocytes Cancer Vaccines Immunotherapy Adoptive Article Mice Lymphopenia medicine Immunology and Allergy Animals Antigen-presenting cell Lymph node Cyclophosphamide Pharmacology business.industry Melanoma Liver Neoplasms Dendritic cell Immunotherapy Dendritic Cells medicine.disease Combined Modality Therapy Mice Inbred C57BL medicine.anatomical_structure Cancer research Female Lymph Nodes business Immunosuppressive Agents Vidarabine |
| Zdroj: | Journal of immunotherapy (Hagerstown, Md. : 1997). 32(6) |
| ISSN: | 1537-4513 |
| Popis: | Treatment of C57BL/6 mice with cyclophosphamide (100 mg/kg) and fludarabine (200 mg/kg) mduced nonmyeloablative lymphodepletion without inhibiting D5 melanoma tumor growth. Using this model, we found that induction of lymphopenia before adoptive transfer of ex vivo anti-CD3/CD28 activated and interleukin-2 expanded D5-G6 tumor draining lymph node cells enhanced the antitumor efficacy of the infused cells in both pulmonary metastases and subcutaneous D5 bearing mice. However, induction of lymphopenia did not promote intratumoral or extratumoral proliferation or accumulation of the infused cells. We have previously shown that radiotherapy enhances the therapeutic efficacy of intratumoral unpulsed dendritic cell vaccination in subcutaneous murine tumor models by augmenting the induction of antitumor cellular immune responses. Here, we confirmed this finding in a murine metastatic melanoma liver tumor model. Furthermore, local tumor irradiation combined with intratumoral dendritic cell administration significantly enhanced the therapeutic efficacy of tumor-reactive T cell adoptive transfer in this lymphodepleted liver tumor model. This was evident by reduced liver tumor size, decreased incidence of spontaneous intra-abdominal metastasis, and prolonged survival, resulting in 46% of mice cured. This enhanced antitumor activity was associated with a selective increase in proliferation, accumulation, and function of CD4 + rather than CD8 + infused cells. This multi-modality regimen may have translational applications for the treatment of human cancers. |
| Databáze: | OpenAIRE |
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