[Study on extraction and immune regulation of polysaccharides from Marsdenia tenacissima]
| Autor: | Yonghua Yue, Xuliang Hao, Yuanyuan Li, Li Xianrong, Xiufeng Zhu |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
medicine.medical_treatment Intraperitoneal injection CD4-CD8 Ratio Marsdenia tenacissima Biology Chemical Fractionation Polysaccharide Mice Immune system Polysaccharides medicine Animals Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Sugar chemistry.chemical_classification Chromatography Interleukin-6 Extraction (chemistry) Body Weight Immune regulation Temperature Marsdenia Peripheral blood Complementary and alternative medicine chemistry Interleukin-2 Spleen |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 37(4) |
| ISSN: | 1001-5302 |
| Popis: | OBJECTIVE To optimize the extraction technology and determine the immune activity of polysaccharides from Marsdenia tenacissima. METHOD The optimum extraction technology of polysaccharide from M. tenacissima was detected by orthogonal experiments with the extraction rate of polysacchride and the total sugar content as indicators. The immunocompromised mice model was established by intraperitoneal injection cyclophosphamide to detected the content of IL-2, IL-6 in serum, CD4+, CD8+ in the peripheral blood by ELISA and flow cytometry, respectively. RESULT By the extraction rate of polysaccharide, the sequence of seriousness of all affecting factors from high to low was extracting times, temperature, heating time and water ratio. By the total sugar content, the sequence was temperature, extracting times, water ratio and heating time. Compared with the model group, the pleen index, IL-2, IL-6, CD4/CD8+ were increased significantly (P < 0.05) in the 0.14 g x kg(-1) group and 0. 28 g x kg(-1) group. CONCLUSION T he optimum extraction condition was as follows: extraction three times/1.5 hours at 100 degrees C with 1:8 ratio of M. tenacissima to water. The polysaccharide of M. tenacissima can enhance the cellular immune and humoral immune. |
| Databáze: | OpenAIRE |
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