Mycobacterium tuberculosis peptide E7/HLA-DRB1 tetramers with different HLA-DR alleles bound CD4+ T cells might share identical CDR3 region
| Autor: | Jiao Wang, Tao Chen, Xi Huang, Lin Zhou, Yaoju Tan, Xiaomin Lai, Yanming Shen, Xiaoxin Tu, Jianxiong Liu, Yimin Fang, Kouxing Zhang, Yanan Yao, Cong Wang, Yichuan Gan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine CD3 Complex Receptors Antigen T-Cell alpha-beta T cell lcsh:Medicine chemical and pharmacologic phenomena Complementarity determining region Article Mycobacterium tuberculosis 03 medical and health sciences 0302 clinical medicine Immune system Antigen HLA-DR medicine Humans Tuberculosis lcsh:Science HLA-DRB1 Alleles Antigens Bacterial Multidisciplinary biology lcsh:R T-cell receptor hemic and immune systems biology.organism_classification Complementarity Determining Regions Molecular biology 030104 developmental biology medicine.anatomical_structure lcsh:Q HLA-DRB1 Chains Molecular Chaperones 030215 immunology |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Human CD4+ T cells play an important role in the immune response to Mycobacterium tuberculosis (MTB). However, little is known about the spectratyping characteristics of the CD4+ T-cell receptor (TCR) α- and β-chains CDR3 region in tuberculosis (TB) patients. We sorted MTB peptide E7-bound CD4+ T cells by using E7/HLA-DR tetramers constructed with different HLA-DRB1 alleles and extracted the CDR3 amino-acid sequences of TCR α- and β-chains. The results showed that the CDR3 sequences of E7-bound CD4+ T cells were completely or partially identical in a single patient. The sequences of MTB peptide C5-bound CD4+ T cells shared another, and non-peptide bound CD4+ T cells, as well as unbound CD4+ T cells with tetramers were different from each other. Specifically, diverse CDR3 sequences of E7-bound CD4+ T cells displayed similar protein tertiary structure in one TB patient. In summary, the TCR α- and β-chains of CDR3 lineage of CD4+ T cells in TB patients apparently drifted, and the predominant CDR3 sequences of TCR α- and β-chains that recognized the MTB antigen exhibited peptide specificity, and certain HLA-DR restriction was also established. This study elucidates the possible causes and mechanisms of peptide-specific CD4+ T-cell-related presentation against MTB. |
| Databáze: | OpenAIRE |
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