Retinoic acid stimulates growth hormone synthesis in human somatotrophic adenoma cells: Characterization of its nuclear receptors
| Autor: | Jean Guibourdenche, Charlotte Djakouré, P. Pagesy, Danièle Evain-Brion, F. Peillon, Cécile Rochette-Egly, J. Y. Li, Dominique Porquet |
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| Rok vydání: | 1997 |
| Předmět: |
Adenoma
Adult Male medicine.medical_specialty Somatotropic cell Receptors Retinoic Acid Blotting Western Cell Retinoic acid Tretinoin Biochemistry chemistry.chemical_compound Internal medicine medicine Humans Pituitary Neoplasms RNA Messenger Molecular Biology Cells Cultured biology Human Growth Hormone Cell Biology Middle Aged Growth hormone secretion Retinoic acid receptor Retinoid X Receptors medicine.anatomical_structure Endocrinology Nuclear receptor chemistry Polyclonal antibodies Acromegaly biology.protein Female Intracellular Transcription Factors |
| Zdroj: | Journal of Cellular Biochemistry. 65:25-31 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/(sici)1097-4644(199704)65:1<25::aid-jcb3>3.0.co;2-0 |
| Popis: | In order to gain a better understanding on the possible role of retinoic acid (RA) on human GH secretion, we have characterized the expression of its nuclear receptors in somatotropic adenoma cell extracts. By immunoblotting with rabbit polyclonal antibodies directed against RARα, β, and γ and RXRα and β, we could only detect the presence of RARα and RXRα proteins. The predominant expression of RXRα was confirmed at the mRNA level by Northern and slot-blot analysis. When then investigated the effect of RA on GH synthesis in cell culture of adenomatous somatotrophs. In cultured cells, RA (1 μM) stimulated GH secretion, increased intracellular GH content and GH mRNA levels within 72 h, suggesting a modulation of GH synthesis by RA. J. Cell. Biochem 65:25–31. © 1997 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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