Permeabilization of the plasma membrane of L1210 mouse leukemia cells using lithotripter shock waves
| Autor: | S. Gambihler, Michael Delius, J. W. Ellwart |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cell Membrane Permeability
Time Factors Physiology Biophysics Flow cytometry chemistry.chemical_compound Mice In vivo Lithotripsy medicine Tumor Cells Cultured Animals Leukemia L1210 medicine.diagnostic_test Molecular mass Cell Membrane Dextrans Cell Biology Cell sorting Flow Cytometry Molecular biology In vitro Kinetics Dextran chemistry L1210 cells Intracellular Fluorescein-5-isothiocyanate |
| Zdroj: | The Journal of membrane biology. 141(3) |
| ISSN: | 0022-2631 |
| Popis: | Permeabilization of L1210 cells by lithotripter shock waves in vitro was monitored by evaluating the accumulation of fluorescein-labeled dextrans with a relative molecular mass ranging from 3,900–2,000,000. Incubation with labeled dextran alone caused a dose- and time-dependent increase in cellular fluorescence as determined by flow cytometry, with a vesicular distribution pattern in the cells consistent with endocytotic uptake. Shock wave exposure prior to incubation with labeled dextran revealed similar fluorescence intensities compared to incubation with labeled dextran alone. When cells were exposed to shock waves in the presence of labeled dextran, mean cellular fluorescence was further increased, indicating additional internalization of the probe. Confocal laser scanning microscopy confirmed intracellular fluorescence of labeled dextran with a diffuse distribution pattern. Fluorescence-activated cell sorting with subsequent determination of proliferation revealed that permeabilized cells were viable and able to proliferate. Permeabilization of the membrane of L1210 cells by shock waves in vitro allowed loading of dextrans with a relative molecular mass up to 2,000,000. Permeabilization of tumor cells by shock waves provides a useful tool for introducing molecules into cells which might be of interest for drug targeting in tumor therapy in vivo. |
| Databáze: | OpenAIRE |
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