Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Autor:	Louise E. Bird, J.M. Diprose, Raymond J. Owens, Heather Rada, John G. Flanagan, Robert J.C. Gilbert
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	Genetics Cloning Fusion Expression vector Pcr cloning Biology medicine.disease_cause law.invention Transformation (genetics) chemistry.chemical_compound chemistry law medicine Escherichia coli Polymerase chain reaction DNA
Zdroj:	DNA Cloning and Assembly Methods ISBN: 9781627037631
DOI:	10.1007/978-1-62703-764-8_15
Popis:	In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. © Springer Science+Business Media New York 2014.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0582a7cb8ef67f9cabe1de68757f015 https://doi.org/10.1007/978-1-62703-764-8_15 Zobrazit plný text záznamu