Epinephrine-entrapped chitosan nanoparticles covered by gelatin nanofibers: A bi-layer nano-biomaterial for rapid hemostasis
| Autor: | Amir Amani, Mahboubeh Atashgahi, Behnaz Ghaemi, Alireza Valizadeh, Mohammad Hossein Nekoofar, Arfa Moshiri |
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| Rok vydání: | 2021 |
| Předmět: |
Hemostatic Agent
Chitosan Hemostasis food.ingredient Epinephrine Chemistry Nanofibers Pharmaceutical Science Biomaterial Biocompatible Materials Gelatin Rats Rats Sprague-Dawley food Nanofiber Coagulation testing Coagulation (water treatment) Animals Nanoparticles Biomedical engineering Blood coagulation test |
| Zdroj: | International journal of pharmaceutics. 608 |
| ISSN: | 1873-3476 |
| Popis: | Uncontrolled hemorrhage accounts for significant death risk both in trauma and surgery. Various bleeding control techniques have been emerged to augment hemostasis, which still has several limitations and drawbacks. In this study, epinephrine-entrapped chitosan nanoparticles were electrosprayed on a base pad and covered by a gelatin nanofiber layer (E-CS-Gl. Physico-chemical characteristics, hemocompatibility, cytotoxicity, and blood coagulation tests were studied in-vitro, and blood coagulation and hemostasis potential tests were performed in-vivo. The in-vitro results showed that the prepared nano-biomaterial is cytocompatible against HuGu cells. Also, hemocompatibility studies showed that PT and aPTT times did not change in comparison with the controls. Further blood coagulation study indicated that E-CS-Gl provides an ultimate interface to induce red blood cell absorption and aggregation, resulting in augmented blood coagulation. E-CS-Gl also caused rapid clotting in rat models of ruptured femoral artery and liver compared to controls. Findings exhibited that E-CS-Gl is a safe and effective hemostatic agent and provides a new approach for fast and safe hemorrhage control. |
| Databáze: | OpenAIRE |
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