Elastography of multicellular spheroids using 3D light microscopy
| Autor: | Devina Jaiswal, Kazunori Hoshino, Kevin P. Claffey, Yuji Tomizawa, Zoe M. Moscato |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Microscope Materials science medicine.diagnostic_test Confocal Spheroid 01 natural sciences Atomic and Molecular Physics and Optics Article law.invention 010309 optics 03 medical and health sciences Differential interference contrast microscopy law Indentation 0103 physical sciences Microscopy embryonic structures medicine Elastography Deformation (engineering) 030304 developmental biology Biotechnology Biomedical engineering |
| Zdroj: | Biomedical optics express. 10(5) |
| ISSN: | 2156-7085 |
| Popis: | We have demonstrated a new method of 3D elastography based on 3D light microscopy and micro-scale manipulation. We used custom-built micromanipulators to apply a mechanical force onto multicellular tumor spheroids (200-300 µm in size) and recorded the induced compression with a differential interference contrast (DIC)/confocal microscope to obtain a 4D (x, y, z, and indentation steps) image sequence. Deformation analysis made through 3D pattern tracking without using fluorescence revealed 3D structural and spatial heterogeneity in tumor spheroids. We observed a 20-30 µm-sized spot of locally-induced large deformation within a tumor spheroid. We also found solid fibroblast cores formed in a tumor-fibroblast co-culture spheroid to be stiffer than surrounding cancer cells, which would not have been discovered using only conventional fluorescence. Our new method of 3D elastography may be used to better understand structural composition in multicellular spheroids through analysis of mechanical heterogeneity. |
| Databáze: | OpenAIRE |
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