Imaging Atherosclerotic Plaque Inflammation via Folate Receptor Targeting Using a Novel F-18-Folate Radiotracer
| Autor: | Philipp A. Kaufmann, Cristina Müller, Adrienne Müller, Zoran Rancic, Stefanie-Dorothea Krämer, Thomas Betzel, Katharina Beck, Cindy R. Fischer, Simon M. Ametamey, Roger Schibli |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty CD68 Biomedical Engineering Biology Condensed Matter Physics In vitro law.invention Blot law Folate receptor Gene expression medicine Molecular Medicine Immunohistochemistry Radiology Nuclear Medicine and imaging Polymerase chain reaction Biotechnology Plaque inflammation |
| Zdroj: | Molecular Imaging, 13 (2) |
| ISSN: | 1535-3508 1536-0121 |
| Popis: | Folate receptor β (FR-β) is overexpressed on activated, but not resting, macrophages involved in a variety of inflammatory and autoimmune diseases. A pivotal step in atherogenesis is the subendothelial accumulation of macrophages. In nascent lesions, they coordinate the scavenging of lipids and cellular debris to define the likelihood of plaque inflammation and eventually rupture. In this study, we determined the presence of FR-β-expressing macrophages in atherosclerotic lesions by the use of a fluorine-18-labeled folate-based radiotracer. Human endarterectomized specimens were used to measure gene expression levels of FR-β and CD68. Increased FR-β and CD68 levels were found in atherosclerotic plaques compared to normal artery walls by quantitative real-time polymerase chain reaction. Western blotting and immunohistochemistry demonstrated prominent FR-β protein levels in plaques. FR- β-positive cells colocalized with activated macrophages (CD68) in plaque tissue. Carotid sections incubated with 3′-aza-2′- [18F]fluorofolic acid displayed increased accumulation in atherosclerotic plaques through in vitro autoradiography. Specific binding of the radiotracer correlated with FR-β-expressing macrophages. These results demonstrate high FR-β expression in atherosclerotic lesions of human carotid tissue correlating with CD68-positive macrophages. Areas of high 3′-aza-2′-[18F]fluorofolic acid binding within the lesions represented FR-β-expressing macrophages. Selectively targeting FR-β-positive macrophages through folate-based radiopharmaceuticals may be useful for noninvasive imaging of plaque inflammation. Molecular Imaging, 13 (2) ISSN:1535-3508 ISSN:1536-0121 |
| Databáze: | OpenAIRE |
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