Distribution and Risk of Mycolactone-Producing Mycobacteria Transmission within Buruli Ulcer Endemic Communities in Côte d'Ivoire
| Autor: | Lydia Mosi, Bassirou Bonfoh, Charles A. Narh, Christelle Dassi, Charles Quaye, Joseph Allico Djaman, Danièle O Konan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Buruli ulcer 030106 microbiology Biology law.invention Microbiology 03 medical and health sciences chemistry.chemical_compound law Genotype medicine Typing Mycolactone Polymerase chain reaction General Immunology and Microbiology Public Health Environmental and Occupational Health medicine.disease biology.organism_classification 16S ribosomal RNA Virology 3. Good health Variable number tandem repeat Infectious Diseases chemistry Mycobacterium ulcerans mycolactone-producing mycobacteria environment human transmission VNTR typing phylogenetics |
| Zdroj: | Tropical Medicine and Infectious Disease; Volume 2; Issue 1; Pages: 3 |
| ISSN: | 2414-6366 |
| Popis: | In Buruli ulcer (BU) endemic communities, most mycolactone-producing mycobacteria (MPM), including Mycobacterium ulcerans, the causative agent, are present in water bodies used by inhabitants; yet, their mode of transmission is still unclear. This study aimed to assess the distribution of MPM strains, both from human suspected cases and aquatic environments, for identifying possible transmission modes within two BU endemic districts, Daloa and Tiassalé (Taabo), in Côte d’Ivoire. Collected samples were processed using conventional polymerase chain reaction and screened for the presence of non-tuberculous mycobacteria (NTM) and MPMs using 16S rRNA, IS2404 and enoyl reductase (ER) primers. MPM-positive samples were further discriminated using variable number tandem repeat (VNTR) typing and sequencing. 16S rRNA and IS2404 sequences confirmed that 94% of the clinical samples contained MPMs. For environmental samples, 53% were contaminated with NTMs, of which 17% contained MPMs particularly M. ulcerans, suggesting that water-related activities could predispose inhabitants to BU transmission. MPM discrimination by VNTR at four M. ulcerans Agy99 loci identified genotype C, previously reported in Côte d’Ivoire as the most dominant profile. Phylogenetic clustering on the basis of genetic diversity in the MIRU 1 locus showed two main M. ulcerans lineages in Côte d’Ivoire. |
| Databáze: | OpenAIRE |
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