Murine Cytomegalovirus Major Immediate-Early Enhancer Region Operating as a Genetic Switch in Bidirectional Gene Pair Transcription
| Autor: | Matthias J. Reddehase, Natascha K. A. Grzimek, Birgit Kühnapfel, Christian O. Simon |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Mice Inbred BALB C Base Sequence Transcription Genetic General transcription factor Immunology Response element Cytomegalovirus Enhancer RNAs E-box Promoter Biology Microbiology Genome Replication and Regulation of Viral Gene Expression Mice Enhancer Elements Genetic Virology Insect Science TAF2 Animals Enhancer trap Enhancer Genes Immediate-Early DNA Primers |
| Zdroj: | Journal of Virology. 81:7805-7810 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.02388-06 |
| Popis: | Enhancers are defined as DNA elements that increase transcription when placed in any orientation relative to a promoter. The major immediate-early (MIE) enhancer region of murine cytomegalovirus is flanked by transcription units ie1/3 and ie2 , which are transcribed in opposite directions. We have addressed the fundamental mechanistic question of whether the enhancer synchronizes transcription of the bidirectional gene pair (synchronizer model) or whether it operates as a genetic switch, enhancing transcription of either gene in a stochastic alternation (switch model). Clonal analysis of cytokine-triggered, transcription factor-mediated MIE gene expression from latent viral genomes provided evidence in support of the switch model. |
| Databáze: | OpenAIRE |
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