A Conserved Role of the Unconventional Myosin 1d in Laterality Determination
| Autor: | Franziska Fuhl, Sabrina Kurz, Stéphane Noselli, Tim Ott, Melanie Tingler, Axel Schweickert, Markus Maerker, Janine M. LeBlanc-Straceski, Martin Blum |
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| Přispěvatelé: | University of Hohenheim, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Morpholino [SDV]Life Sciences [q-bio] Xenopus Nodal signaling Myosins Xenopus Proteins General Biochemistry Genetics and Molecular Biology Xenopus laevis 03 medical and health sciences 0302 clinical medicine Morphogenesis Animals ComputingMilieux_MISCELLANEOUS Body Patterning biology Convergent extension Wnt signaling pathway Cell Polarity Gene Expression Regulation Developmental Morphant Gastrula biology.organism_classification Cell biology Gastrulation 030104 developmental biology General Agricultural and Biological Sciences NODAL 030217 neurology & neurosurgery |
| Zdroj: | Current Biology-CB Current Biology-CB, Elsevier, 2018, 28 (5), pp.810-816.e3. ⟨10.1016/j.cub.2018.01.075⟩ |
| ISSN: | 0960-9822 1879-0445 |
| DOI: | 10.1016/j.cub.2018.01.075 |
| Popis: | Summary Anatomical and functional asymmetries are widespread in the animal kingdom [1, 2]. In vertebrates, many visceral organs are asymmetrically placed [3]. In snails, shells and inner organs coil asymmetrically, and in Drosophila , genitalia and hindgut undergo a chiral rotation during development. The evolutionary origin of these asymmetries remains an open question [1]. Nodal signaling is widely used [4], and many, but not all, vertebrates use cilia for symmetry breaking [5]. In Drosophila , which lacks both cilia and Nodal, the unconventional myosin ID ( myo1d ) gene controls dextral rotation of chiral organs [6, 7]. Here, we studied the role of myo1d in left-right (LR) axis formation in Xenopus . Morpholino oligomer-mediated myo1d downregulation affected organ placement in >50% of morphant tadpoles. Induction of the left-asymmetric Nodal cascade was aberrant in >70% of cases. Expression of the flow-target gene dand5 was compromised, as was flow itself, due to shorter, fewer, and non-polarized cilia at the LR organizer. Additional phenotypes pinpointed Wnt/planar cell polarity signaling and suggested that myo1d , like in Drosophila [8], acted in the context of the planar cell polarity pathway. Indeed, convergent extension of gastrula explant cultures was inhibited in myo1d morphants, and the ATF2 reporter gene for non-canonical Wnt signaling was downregulated. Finally, genetic interference experiments demonstrated a functional interaction between the core planar cell polarity signaling gene vangl2 and myo1d in LR axis formation. Thus, our data identified myo1d as a common denominator of arthropod and chordate asymmetry, in agreement with a monophyletic origin of animal asymmetry. |
| Databáze: | OpenAIRE |
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