Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L
| Autor: | Li-Quan Zhu, Qianying Liu, Siru Zhou, Qinqin Xie, Yizhong Zhang, Tonghong Zuo, Dengke Hu, Yimei Liu, Hecui Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
family expansion
GAPC Down-Regulation Brassica Flowers QH426-470 self-incompatibility Article Chromosomes Plant Evolution Molecular Transcriptome Gene Expression Regulation Plant Stress Physiological Gene expression B. oleracea L Genetics Cloning Molecular GAPDH Pollination Gene Conserved Sequence Genetics (clinical) Glyceraldehyde 3-phosphate dehydrogenase Plant Proteins biology Gene Expression Profiling Chromosome Mapping Glyceraldehyde-3-Phosphate Dehydrogenases cloning and expression analysis Promoter biology.organism_classification Subcellular localization Cell biology Molecular Weight Protein kinase domain Multigene Family biology.protein Brassica oleracea |
| Zdroj: | Genes, Vol 12, Iss 1719, p 1719 (2021) Genes Volume 12 Issue 11 |
| ISSN: | 2073-4425 |
| Popis: | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in Brassica oleracea. In this study, we performed a genome-wide identification of BoGAPDH in B. oleracea and performed cloning and expression analysis of one of the differentially expressed genes, BoGAPC. A total of 16 members of the BoGAPDH family were identified in B. oleracea, which were conserved, distributed unevenly on chromosomes and had tandem repeat genes. Most of the genes were down-regulated during self-pollination, and the highest expression was found in stigmas and sepals. Different transcriptome data showed that BoGAPDH genes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed gene BoGAPC and found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the stigma, which was consistent with the result of GUS staining. The promoter region of the gene not only has stress response elements and plant hormone response elements, but also has a variety of specific elements for regulating floral organ development. Subcellular localization indicates that the BoGAPC protein is located in the cytoplasm and belongs to the active protein in the cytoplasm. The results of prokaryotic expression showed that the size of the BoGAPC protein was about 37 kDa, which was consistent with the expected results, indicating that the protein was induced in prokaryotic cells. The results of yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted with the BoGAPC protein. The above results suggest that the BoGAPDH family of B. oleracea plays an important role in the process of plant stress resistance, and the BoGAPC gene may be involved in the process of self-incompatibility in B. oleracea, which may respond to SI by encoding proteins directly interacting with SRK. |
| Databáze: | OpenAIRE |
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