Recharacterization of fungal dinucleoside polyphosphate (HS3)
| Autor: | Peter C. Loewen, David R. McNaughton, Herb B. LéJohn, Glen R. Klassen |
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| Rok vydání: | 1978 |
| Předmět: |
Chemical Phenomena
Oligonucleotide Chemistry Polyphosphate Fungi Oligonucleotides Amino Sugars Uracil General Medicine Uridine Diphosphate Phosphates Adenosine Diphosphate Adenosine diphosphate chemistry.chemical_compound Uridine diphosphate Glutamates Oomycetes Biochemistry medicine Mannitol medicine.drug |
| Zdroj: | Canadian Journal of Biochemistry. 56:217-226 |
| ISSN: | 0008-4018 |
| Popis: | Three polyphosphorylated dinucleosides given the pseudonyms of HS3, HS2, and HS1 that were erroneously described as diguanosine polyphosphates (LéJohn, H. B., Cameron, L. E., McNaughton, D. R. &Klassen, G. R. (1975) Biochem. Biophys. Res. Commun. 66, 460–467) have been repurified and partially recharacterized. They have proved to be extremely complex molecules; chemical (HCl and KOH hydrolysis), physical (ultraviolet-light spectral analysis and ion-exchange chromatography), and enzymic (nucleotide pyrophosphatase and bacterial alkaline phosphatase hydrolysis) studies showed that (i) all three HS compounds are uracil rich and (ii) only HS3 contains a purine nucleoside and glutamate. The partial structure of HS3 was deciphered as a moiety of ADP – sugar X – glutamate (the mode of attachment of glutamate is obscure) that is covalently linked to another moiety composed of UDP, mannitol, and four phosphates. Sugar X had chromatographic characteristics of ribitol, but the chromatographic isolate also contained a ninhydrin-sensitive entity presumed to be an amino group. Sugar X, therefore, may be an amino sugar polyol. Only the general chemical compositions of HS2and HS1 were determined. Each contained two uridines and HS2 had 10 phosphates whereasHS1 had 12. |
| Databáze: | OpenAIRE |
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