Original Research: Establishment of an early embolus-related cerebral injury model after cardiopulmonary bypass in miniature pigs
| Autor: | Haiyu Chen, Weiwei Zhang, Guoxing Weng, Shun Yu, Huan Wang, Jiayin Bao, Xiying Cao, Zhi Dou, Min Li |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Miniature pig Swine Neuroimaging 030204 cardiovascular system & hematology General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences symbols.namesake 0302 clinical medicine Embolus law medicine Cardiopulmonary bypass Animals cardiovascular diseases Cardiopulmonary Bypass biology medicine.diagnostic_test Intracranial Embolism business.industry Penumbra Brain Magnetic resonance imaging biology.organism_classification Surgery Disease Models Animal Diffusion Magnetic Resonance Imaging surgical procedures operative Brain Injuries Anesthesia Nissl body symbols Swine Miniature medicine.symptom business 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Experimental Biology and Medicine. 241:1819-1824 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/1535370216648804 |
| Popis: | Embolus-related cerebral injury is still a serious adverse event after cardiopulmonary bypass (CPB). But there is no stable animal model for basic and clinical research purposes. We chose miniature pig to establish a stable animal model of embolus-related cerebral injury after CPB and verified the validity of results by correlating the histopathological findings with those of diffusion-weighted magnetic resonance imaging (DW-MRI). Based on different treatment regimens, 24 male miniature pigs were randomly assigned into four groups: Control, CPB, embolus, and CPB–embolus groups. DW-MRI was performed before and after surgery to diagnose and locate the brain lesions. Histopathological changes in brain tissues were examined using H&E and Nissl staining. All surgical procedures were uneventful with 100% postoperative survival of pigs. Two animals in the Embolus group and six animals in the CPB–embolus group showed signs of ischemic penumbra on DW-MRI performed 6 h after surgery. Consistent with the results of DW-MRI, histopathological examination showed necrosis and ischemic lesions. In this paper, we demonstrate the feasibility and validity of a pig model of embolus-related cerebral injury associated with CPB. This model may be used in the future for basic and translational research. |
| Databáze: | OpenAIRE |
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