Mechanistic toxicity of DEHP at environmentally relevant concentrations (ERCs) and ecological risk assessment in the Three Gorges Reservoir Area, China
Autor: | Wei-Guo Li, Yu-Mei Tang, De-Sheng Pei, Phyllis R. Strauss, Pan-Pan Jia, Wen-Xu Xiong, Muhammad Junaid, Hai-Yang Huang |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
China endocrine system DNA damage Health Toxicology and Mutagenesis Phthalic Acids 010501 environmental sciences Pharmacology Toxicology Risk Assessment 01 natural sciences Phosphatidylinositol 3-Kinases 03 medical and health sciences chemistry.chemical_compound Diethylhexyl Phthalate Toxicity Tests Animals Bioassay Viability assay Zebrafish 0105 earth and related environmental sciences Ecology biology TOR Serine-Threonine Kinases Phthalate General Medicine Aryl hydrocarbon receptor biology.organism_classification Pollution 030104 developmental biology chemistry Toxicity Cancer cell biology.protein Environmental Pollutants DNA Damage |
Zdroj: | Environmental Pollution. 242:1939-1949 |
ISSN: | 0269-7491 |
Popis: | Di-(2-ethylhexyl) phthalate (DEHP) associated in vitro/vivo toxicity at current environmentally relevant concentration (ERC) with attendant ecological risks in the Three Gorges Reservoir Area (TGRA) is still elusive. Responding to this challenge, a novel integrated study based on analytical and biological assays was designed to elucidate the underlying mechanisms for toxicity of DEHP and its ecological risks at ERC. In this study, GC-MS analysis showed that the highest environmental concentration of DEHP in the TGRA surface water was nearly double that of WHO and USEPA standards. Both distribution and ecological risk decreased from the upper to middle and lower reaches of the TGRA. In vitro toxicity was assessed by cell viability and DNA damage assays: DEHP exposure at ERCs (100–800 μg/L) caused significant reduction in cell viability and elevated DNA damage. Further, DEHP exposure above 400 μg/L resulted in enhanced migration behavior of cancer cells. For in vivo toxicity assessment, short term acute exposure (7 d, 400 μg/L) apparently activated the PI3K-AKT-mTOR pathway, and chronic low-level exposure (3 months, 10–33 μg/L) suppressed the hypothalamus pituitary thyroid (HPT) axis pathway in zebrafish. In addition, acute low-level exposure (5 d, 33–400 μg/L) to DEHP increased aryl hydrocarbon receptor (AhR) activity in Tg(cyp1a:gfp) zebrafish in a concentration-dependent manner. In short, DEHP at ERC has extended potential to induce diverse in vitro and in vivo toxicity at concentrations that also cause impairment of biochemical function in aquatic species of the TGRA. |
Databáze: | OpenAIRE |
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