Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety 'Tellahamsa'

Autor: Ch V Durga Rani, C. Cheralu, V Bhuvaneshwari, D Sanjeev Rao, S Krishnam Raju, G Swathi, Ch. Anuradha, E. Ramprasad, D Mahesh, Chakravarthy Rajan, R. Jagadeeswar, M. S. Prasad, K. N. Yamini, Jamaloddin, Gouri Sankar Laha, P. Sravanthi, K. Aruna Kumari, S Vanisri, Raman Meenakshi Sundaram, G Rajani, N. Arun Prem Kumar, M. Sheshu Madhav, P.V. Satyanarayana
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Genetic Screens
Rice Blast Fungus
Introgression
Gene Identification and Analysis
Selection Markers
Plant Science
01 natural sciences
Genome
Geographical Locations
Genotype
Bacterial blight
Flowering Plants
Disease Resistance
Genetics
Multidisciplinary
Plant Anatomy
Plant Fungal Pathogens
Eukaryota
food and beverages
Plants
Experimental Organism Systems
Medicine
Genome
Plant
Research Article
Genetic Markers
Inflorescences
Evolutionary Processes
Asia
DNA
Plant
Science
Plant Pathogens
India
Biology
Research and Analysis Methods
Mega
03 medical and health sciences
Plant and Algal Models
Grasses
Panicles
Molecular Biology Techniques
Molecular Biology
Gene
Plant Diseases
Evolutionary Biology
Organisms
Fungi
Biology and Life Sciences
Oryza
Marker Genes
Plant Pathology
Biotic stress
030104 developmental biology
Genetic marker
People and Places
Backcrossing
Animal Studies
Rice
Edible Grain
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 6, p e0234088 (2020)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0234088
Popis: Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a well-adapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F1 to ICF3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC2F1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.
Databáze: OpenAIRE
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