Identification of discrete, intermingled hypocretin neuronal populations
| Autor: | Bernhard Klingenberg, Manasi Iyer, Rachel A. Essner, Matthew E. Carter |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Hypothalamus Neuropeptide Nucleus accumbens Biology Article 03 medical and health sciences Mice 0302 clinical medicine Neural Pathways medicine Animals Neurons Orexins General Neuroscience Orexin Ventral tegmental area Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Locus coeruleus Wakefulness Nucleus Neuroscience 030217 neurology & neurosurgery psychological phenomena and processes |
| Zdroj: | The Journal of comparative neurology. 526(18) |
| ISSN: | 1096-9861 |
| Popis: | Neurons in the lateral hypothalamic area that express hypocretin (Hcrt) neuropeptides help regulate many behaviors including wakefulness and reward seeking. These neurons project throughout the brain, including to neural populations that regulate wakefulness, such as the locus coeruleus (LC) and tuberomammilary nucleus (TMN), as well as to populations that regulate reward, such as the nucleus accumbens (NAc) and ventral tegmental area (VTA). To address the roles of Hcrt neurons in seemingly disparate behaviors, it has been proposed that Hcrt neurons can be anatomically subdivided into at least two distinct subpopulations: a "medial group" that projects to the LC and TMN, and a "lateral group" that projects to the NAc and VTA. Here, we use a dual retrograde tracer strategy to test the hypotheses that Hcrt neurons can be classified based on their downstream projections and medial/lateral location within the hypothalamus. We found that individual Hcrt neurons were significantly more likely to project to both the LC and TMN or to both the VTA and NAc than would be predicted by chance. In contrast, we found that Hcrt neurons that projected to the LC or TMN were mostly distinct from Hcrt neurons that projected to the VTA or NAc. Interestingly, these two populations of Hcrt neurons are intermingled within the hypothalamus and cannot be classified into medial or lateral groups. These results suggest that Hcrt neurons can be distinguished based on their downstream projections but are intermingled within the hypothalamus. |
| Databáze: | OpenAIRE |
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