Characterization of genes responsive to osmotic and oxidative stresses of the sugarcane bacterial pathogen Leifsonia xyli subsp. xyli
| Autor: | Luis Eduardo Aranha Camargo, Claudia Barros Monteiro-Vitorello, Ricardo A. Azevedo, Raphael Severo da Cunha Antunes de Faria, Mariana Cicarelli Cia |
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| Rok vydání: | 2019 |
| Předmět: |
Leifsonia xyli
Microbiology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound RAQUITISMO DAS SOQUEIRAS Osmotic Pressure PEG ratio Gene expression Media Technology Plant Diseases 030304 developmental biology Bacterial Fungal and Virus Molecular Biology - Research Paper chemistry.chemical_classification 0303 health sciences Reactive oxygen species Host Microbial Interactions biology Superoxide Dismutase 030306 microbiology Catalase biology.organism_classification Trehalose Saccharum Actinobacteria Oxidative Stress chemistry Osmolyte biology.protein Lipid Peroxidation |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Braz J Microbiol |
| ISSN: | 1678-4405 1517-8382 |
| DOI: | 10.1007/s42770-019-00163-6 |
| Popis: | Leifsonia xyli subsp. xyli (Lxx) colonizes the xylem vessels of sugarcane, a plant niche where microorganisms are highly exposed to oxidative and osmotic stresses. This study performed an in silico analysis of the genome of Lxx and characterized 16 genes related to the detoxification of oxidative species (peroxidases, O(2)(−) dismutases, and methionine reductases) and to the production and transport of osmolytes and analyzed their expression in vitro after 30, 60, and 120 min of exposure to H(2)O(2) or PEG. The PAGE activity of superoxide dismutase (Mn-SOD as confirmed by inhibition tests) and of catalase (CAT) and the accumulation of trehalose were also assessed. Exposure to H(2)O(2) increased the expression of most oxidative-responsive genes and decreased the expression of those related to osmotic responses, whereas the opposite occurred after exposure to PEG. The isoform profiles of CAT and Mn-SOD shifted in response to H(2)O(2) but not to PEG and Lxx cells accumulated more trehalose over time after exposure to PEG compared with non-exposed cells. The experimental results validated the in silico analysis and indicated that this obligate endophytic parasite has multiple and functional mechanisms to combat the stresses imposed by its host. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-019-00163-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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