DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy

Autor: Francesca Tassan, Olga Moretti, Alberto Pallavicini, Maria Francesca Borney, Claudio G. Ametrano, Francesca Augusta Gabrielli, Mauro Tretiach, Lucia Muggia, Pierluigi Verardo, Elisa Banchi, Stefania Lazzarin, David Stanković
Přispěvatelé: Banchi, Elisa, Ametrano, Claudio Gennaro, Stanković, David, Verardo, Pierluigi, Moretti, Olga, Gabrielli, Francesca, Lazzarin, Stefania, Borney, Maria Francesca, TASSAN TOFFOLA, Francesca, Tretiach, Mauro, Pallavicini, Alberto, Muggia, Lucia
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Genetics and Molecular Biology (all)
0301 basic medicine
Air Microbiology
lcsh:Medicine
Artificial Gene Amplification and Extension
Pathology and Laboratory Medicine
Biochemistry
DNA barcoding
Polymerase Chain Reaction
Geographical locations
Taxonomic composition
Database and Informatics Methods
Fungal Diversity
Biochemistry
Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)
Fungal Reproduction
Medicine and Health Sciences
lcsh:Science
DNA
Fungal
Mycelium
Data Management
Fungal Pathogens
Multidisciplinary
Geography
Fungal genetics
Eukaryota
High-Throughput Nucleotide Sequencing
Spores
Fungal
Europe
Italy
Medical Microbiology
Taxonomy (biology)
Pathogens
Sequence Analysis
Research Article
Computer and Information Sciences
Bioinformatics
Zoology
Sequence Databases
Mycology
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Species Specificity
DNA
Ribosomal Spacer
DNA Barcoding
Taxonomic
Humans
European Union
Fungal Spores
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Taxonomy
Cell Nucleus
lcsh:R
fungi
Organisms
Fungi
Biology and Life Sciences
Genetic Variation
Allergens
Ascomycetes
Spore
030104 developmental biology
Taxon
Biological Databases
lcsh:Q
People and places
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 3, p e0194489 (2018)
ISSN: 1932-6203
Popis: Fungal spores and mycelium fragments are particles which become and remain airborne and have been subjects of aerobiological studies. The presence and the abundance of taxa in aerobiological samples can be very variable and impaired by changeable climatic conditions. Because many fungi produce mycotoxins and both their mycelium fragments and spores are potential allergens, monitoring the presence of these taxa is of key importance. So far data on exposure and sensitization to fungal allergens are mainly based on the assessment of few, easily identifiable taxa and focused only on certain environments. The microscopic method used to analyze aerobiological samples and the inconspicuous fungal characters do not allow a in depth taxonomical identification. Here, we present a first assessment of fungal diversity from airborne samples using a DNA metabarcoding analysis. The nuclear ITS2 region was selected as barcode to catch fungal diversity in mixed airborne samples gathered during two weeks in four sites of North-Eastern and Central Italy. We assessed the taxonomic composition and diversity within and among the sampled sites and compared the molecular data with those obtained by traditional microscopy. The molecular analyses provide a tenfold more comprehensive determination of the taxa than the traditional morphological inspections. Our results prove that the metabarcoding analysis is a promising approach to increases quality and sensitivity of the aerobiological monitoring. The laboratory and bioinformatic workflow implemented here is now suitable for routine, high-throughput, regional analyses of airborne fungi.
Databáze: OpenAIRE
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