Polyphenols and avenanthramides extracted from oat ( Avena sativa L.) grains and sprouts modulate genes involved in glucose and lipid metabolisms in 3T3 L1 adipocytes
| Autor: | Iza F. Pérez-Ramírez, Ramos-Gómez Minerva, Reynoso-Camacho Rosalía, Magdalena Mendoza-Sánchez, Aparicio-Fernández Xochitl, Ofelia Mora |
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| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
Avena 030309 nutrition & dietetics Biophysics Mice 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology food 3T3-L1 Cells Adipocytes otorhinolaryngologic diseases Animals ortho-Aminobenzoates Food science Chromatography High Pressure Liquid Pharmacology 0303 health sciences ACACA Polyphenols food and beverages Lipid metabolism 04 agricultural and veterinary sciences Cell Biology Lipid Metabolism 040401 food science Glucose chemistry Avenanthramide Polyphenol Apigenin Kaempferol Quercetin Food Science |
| Zdroj: | Journal of Food Biochemistry. 45 |
| ISSN: | 1745-4514 0145-8884 |
| Popis: | This study aimed to evaluate the effect of polyphenol (PE) and avenanthramide (AE) extracts from oat grains (OG) and sprouts (OS) on genes related to glucose and lipid metabolisms in 3T3 L1 adipocytes. The AE-OS exerted the greatest effect on genes involved in glucose metabolism, increasing Glut4, Irs1, and Pi3k expression by 3.0- to 3.9-fold. Conversely, the PE-OS exerted the greatest effect on genes involved in lipid metabolism, decreasing Fasn and Acaca expression by 0.2- to 0.3-fold, and increasing Cpt1a and Acadm expression by 2.7- to 3.0-fold. These effects were mainly related to their high content of avenanthramides A (2p), B (2f), and C (2c), quercetin 3-O-rutinoside, kaempferol, sinapoylquinic acid, and apigenin and luteolin derivatives according to the chemometric analysis. In conclusion, this study demonstrated that oat sprouts extract exerts a greater effect than oat grains on the regulation of genes involved in glucose and lipid metabolisms in adipocytes. PRACTICAL APPLICATIONS: This study demonstrates that polyphenols and avenanthramides extracted from oat (Avena sativa L.) grains and sprouts modulate key genes involved in glucose and lipid metabolisms in adipocytes and that oat sprouts exert a greatest health beneficial effect than oat grains due to their higher content of bioactive compounds. In addition, the chemometric analysis identified the bioactive compounds that can be associated with the beneficial effects of oat grains and sprouts, which can be further used for the identification of oat varieties and oat-derived products with high content of these bioactive compounds and, thus, with high nutraceutical potential. |
| Databáze: | OpenAIRE |
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