DNA amplification in glial cells of progressive multifocal leukoencephalopathy: an image analysis study
| Autor: | Sergi Serrano, Ursula Keysers, Carme Aracil, Aurelio Ariza, Marc Isamat, Jose Luis Mate, José J. Navas-Palacios |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty viruses JC virus Biology Gene mutation medicine.disease_cause Pathology and Forensic Medicine Cellular and Molecular Neuroscience medicine Rosaniline Dyes Humans Feulgen stain Coloring Agents Cell Nucleus Paraffin Embedding Progressive multifocal leukoencephalopathy Gene Amplification Leukoencephalopathy Progressive Multifocal virus diseases General Medicine DNA Cell cycle medicine.disease Aneuploidy Flow Cytometry JC Virus Oligodendrocyte Oligodendroglia medicine.anatomical_structure Neurology Astrocytes Neuroglia Neurology (clinical) Tumor Suppressor Protein p53 Astrocyte |
| Zdroj: | Journal of neuropathology and experimental neurology. 55(6) |
| ISSN: | 0022-3069 |
| Popis: | JC virus (JCV), the agent of progressive multifocal leukoencephalopathy (PML), has been shown by both immunohistochemistry and flow cytometry to be associated with p53 protein stabilization. Since stabilization/inactivation of p53 is associated with the development of genomic instability, abnormal cell DNA contents are to be expected in JCV-infected cells of PML. This work explores that possibility by image analysis evaluation of DNA content in PML-infected oligodendrocytes and bizarre astrocytes. Brain paraffin sections of PML lesions from five adult male patients with the acquired immune deficiency syndrome (AIDS) were treated with the Feulgen technique to obtain a stochiometric staining of DNA and analyzed with a microscope image processor. Inclusion-bearing oligodendrocytes exhibited near tetraploid DNA indices in each of the five cases, whereas atypical astrocytes were in the hypertetraploid range in all cases and were polyploid in four instances. This evidence of DNA amplification in PML glial cells is congruent with the functional abolition of p53 protein in association with JCV infection and lends further support to the role of p53 as a keeper of diploid status and guardian of genomic stability. |
| Databáze: | OpenAIRE |
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