Injection of Flagellin into the Host Cell Cytosol by Salmonella enterica Serotype Typhimurium
| Autor: | Hortensia G. Rolán, Yao Hui Sun, Renée M. Tsolis |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Salmonella typhimurium
Salmonella Genomic Islands medicine.disease_cause Biochemistry Cell Line Microbiology Type three secretion system Mice Cytosol medicine Animals Molecular Biology Innate immune system Host cell cytosol biology Calcium-Binding Proteins Cell Biology biology.organism_classification Immunity Innate Neuronal Apoptosis-Inhibitory Protein Toll-Like Receptor 5 Salmonella enterica TLR5 Salmonella Infections biology.protein bacteria Apoptosis Regulatory Proteins Flagellin Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 282:33897-33901 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.c700181200 |
| Popis: | Bacterial flagellins are potent inducers of innate immunity. Three signaling pathways have been implicated in the sensing of flagellins; these involve toll-like receptor 5 (TLR5) and the cytosolic proteins Birc1e/Naip5 and Ipaf. Although the structural basis of TLR5-flagellin interaction is known, little is known about how flagellin enters the host cell cytosol to induce signaling via Birc1e/Naip5 and Ipaf. Here we demonstrate for the first time the translocation of bacterial flagellin into the cytosol of host macrophages by the vacuolar pathogen, Salmonella enterica serotype Typhimurium. Translocation of flagellin into the host cell cytosol was directly demonstrated using beta-lactamase reporter constructs. Flagellin translocation required the Salmonella Pathogenicity Island 1 Type III secretion system (SPI-1 T3SS) but not the flagellar T3SS. |
| Databáze: | OpenAIRE |
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