Proteome rearrangements after auditory learning: high‐resolution profiling of synapse‐enriched protein fractions from mouse brain

Autor: Thilo Kähne, Constanze I. Seidenbecher, Rainer Pielot, Angela Kolodziej, Wolfgang Tischmeyer, Michael Naumann, Sandra Richter, Karl-Heinz Smalla, Alexander Engler, Eckart D. Gundelfinger, Frank W. Ohl, Daniela C. Dieterich
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
auditory learning
Male
Auditory Pathways
Proteome
Chemical synapse
Biochemistry
metabolism [Cytoskeletal Proteins]
Synapse
Discrimination Learning
Mice
Postsynaptic potential
metabolism [Phosphoproteins]
Phosphoproteomics
phosphoproteomics
Brain
chemical synapse
medicine.anatomical_structure
Neuronal Plasticity & Behavior
Original Article
learning and memory
quantitative mass spectrometry
label-free quantification
Metabolic Networks and Pathways
Signal Transduction
Echoic memory
physiology [Gene Expression Regulation]
Biology
Auditory cortex
03 medical and health sciences
Cellular and Molecular Neuroscience
medicine
Animals
ddc:610
metabolism [Synapses]
metabolism [Proteome]
Phosphoproteins
physiology [Discrimination Learning]
label‐free quantification
Mice
Inbred C57BL
Label-free quantification
Cytoskeletal Proteins
030104 developmental biology
metabolism [Auditory Pathways]
Acoustic Stimulation
Gene Expression Regulation
metabolism [Brain]
Synapses
ORIGINAL ARTICLES
Neuroscience
Zdroj: Journal of Neurochemistry
Journal of neurochemistry 138(1), 124-138 (2016). doi:10.1111/jnc.13636
Journal of neurochemistry, 138(1):13636
ISSN: 1471-4159
0022-3042
DOI: 10.1111/jnc.13636
Popis: Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation of individual synaptic proteins during these processes, much less is known about the complex regulation of synaptic proteomes. Recently, we reported that auditory discrimination learning in mice is associated with a relative down-regulation of proteins involved in the structural organization of synapses in various brain regions. Aiming at the identification of biological processes and signaling pathways involved in auditory memory formation, here, a label-free quantification approach was utilized to identify regulated synaptic junctional proteins and phosphoproteins in the auditory cortex, frontal cortex, hippocampus, and striatum of mice 24 h after the learning experiment. Twenty proteins, including postsynaptic scaffolds, actin-remodeling proteins, and RNA-binding proteins, were regulated in at least three brain regions pointing to common, cross-regional mechanisms. Most of the detected synaptic proteome changes were, however, restricted to individual brain regions. For example, several members of the Septin family of cytoskeletal proteins were up-regulated only in the hippocampus, while Septin-9 was down-regulated in the hippocampus, the frontal cortex, and the striatum. Meta analyses utilizing several databases were employed to identify underlying cellular functions and biological pathways. Data are available via ProteomeExchange with identifier PXD003089. How does the protein composition of synapses change in different brain areas upon auditory learning? We unravel discrete proteome changes in mouse auditory cortex, frontal cortex, hippocampus, and striatum functionally implicated in the learning process. We identify not only common but also area-specific biological pathways and cellular processes modulated 24 h after training, indicating individual contributions of the regions to memory processing. © 2016 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry.
Databáze: OpenAIRE
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