Identification of a multi-component berberine 11-hydroxylase from Burkholderia sp. strain CJ1
| Autor: | Hisashi Takeda, Tomoo Hosoe, Daigo Wakana, Fumihiko Sato, Hinaka Yoshida |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Oxygenase biology 030106 microbiology Organic Chemistry Ranunculaceae Palmatine General Medicine biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Hydroxylation 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Berberine Enzyme chemistry Molecular Biology Bacteria Ferredoxin Biotechnology |
| DOI: | 10.6084/m9.figshare.11797197 |
| Popis: | Berberine (BBR) is a protoberberine alkaloid extracted from plants such as Coptis japonica (Ranunculaceae). In a previous report, we demonstrated the existence of a 11-hydroxylation pathway employed by BBR-utilizing bacteria for metabolism of BBR. In the present study, we report the identification of the genes brhA, brhB, and brhC as encoding a multicomponent BBR 11-hydroxylase in Burkholderia sp. strain CJ1. BrhA is belonging to the Rieske non-heme iron oxygenase (RO) family, a class of enzymes known to catalyze the first step in bacterial aromatic-ring hydroxylation. We further demonstrate that BrhA activity requires BrhB (ferredoxin reductase) and BrhC (ferredoxin) as electron transport chain components. A BLAST search revealed that BrhA exhibits 38% and 33% sequence identity to dicamba O-demethylase (DdmC; AY786443) and chloroacetanilide herbicides N-dealkylase (CndA; KJ461679), respectively. To our knowledge, this work represents the first report of a bacterial oxygenase catalyzing the metabolism of a polycyclic aromatic-ring alkaloid. Abbreviations: BBR: berberine; D-BBR: demethyleneberberine; H-BBR: 11-hydroxyberberine; HD-BBR: 11-hydroxydemethyleneberberine; HDBA: 2-hydroxy-3,4-dimethoxybenzeneacetic acid; PAL: palmatine; H-PAL: 11-hydroxypalmatine; BRU: berberrubine; Fd: ferredoxin; FdR: ferredoxin reductase; ETC: electron transport chain Components and functions of the BBR 11-hydroxylase from Burkholderia sp. Strain CJ1. |
| Databáze: | OpenAIRE |
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