Increased expression of IgE-dependent histamine-releasing factor in endometriotic implants
| Autor: | Masahiko Kuroda, Tetsuya Ohbayashi, Yoshinori Kosugi, Akemi Kameta, Masaomi Takayama, Kiyoshi Mukai, Kosuke Oikawa, Keiichi Isaka |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
T cell Endometriosis Inflammation Biology Pathology and Forensic Medicine Peritoneal cavity Endometrium Mice Internal medicine medicine Biomarkers Tumor Cytochrome P-450 CYP1A1 Macrophage Animals Humans Northern blot Peritoneal Cavity Mice Inbred BALB C Monocyte Tumor Protein Translationally-Controlled 1 3T3 Cells Immunoglobulin E medicine.disease Endocrinology medicine.anatomical_structure Gene Expression Regulation Cancer research Immunohistochemistry Female medicine.symptom |
| Zdroj: | The Journal of pathology. 199(3) |
| ISSN: | 0022-3417 |
| Popis: | A complex network of cytokines mediates the immunoregulatory responses leading to endometriosis. Recent intensive studies suggest that monocyte and T cell chemoattractants contribute to the inflammatory environment of endometriotic implants. The relationship between the inflammation present during endometriosis and the development of endometriotic implants in the peritoneal cavity remains unclear. On the other hand, the association between endometriosis and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) exposure has been discussed in recent years, and our previous results revealed that IgE-dependent histamine-releasing factor (HRF) is inducible by TCDD. The present study aimed to clarify the expression, localization, and function of HRF in endometriosis. Northern blot analysis demonstrated that HRF is overexpressed in endometriotic implants. RT-PCR with Southern blot analysis, however, showed that HRF overexpression was not always accompanied by CYP1A1 induction in endometriotic implants, suggesting that HRF is inducible in endometriosis without exposure to TCDD. HRF is also inducible by macrophage colony-stimulating factor (M-CSF). Immunohistochemistry showed CD68-positive macrophages in the stroma of endometriotic implants, adjacent to regions with prominent HRF accumulation. HRF-overexpressing cells exhibited high implantation efficiency in comparison to control cells when the cells were injected into the peritoneal cavities of nude mice. These results suggest that the accumulation of macrophages in endometriotic implants induces HRF; the overexpression of HRF accelerates the growth of endometriotic implants. |
| Databáze: | OpenAIRE |
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