Purification and Characterization of Antioxidant Peptides from Leukocyte Extract of Crocodylus siamensis
| Autor: | Tinnakorn Theansungnoen, Apisak Dhiravisit, Sompong Thammasirirak, Sakda Daduang, Nualyai Yaraksa |
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| Rok vydání: | 2013 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Bioengineering Biology Tandem mass spectrometry Biochemistry Antioxidants Analytical Chemistry Acetic acid chemistry.chemical_compound Picrates Tandem Mass Spectrometry Leukocytes medicine Animals Amino Acid Sequence chemistry.chemical_classification Alligators and Crocodiles Chromatography Tissue Extracts Elution Biphenyl Compounds Organic Chemistry Free Radical Scavengers biology.organism_classification Amino acid chemistry Crocodylus siamensis Peptides Oxidation-Reduction Homogenization (biology) |
| Zdroj: | The Protein Journal. 33:24-31 |
| ISSN: | 1875-8355 1572-3887 |
| DOI: | 10.1007/s10930-013-9536-8 |
| Popis: | Antioxidant peptides were isolated from the leukocyte extract of the Siamese crocodile, Crocodylus siamensis. Crocodile leukocyte was extracted by a combination of methods including freeze-thawing, acetic acid extraction and homogenization. The peptides in the leukocyte extract were purified by anion exchange chromatography and reversed phase-high performance liquid chromatography. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to evaluate the antioxidant activity of the elution peaks at each purification step. As a result, there were two purified peptides exhibiting strong antioxidant activity in reducing free radicals on DPPH molecules. The amino acid sequences of these peptides were determined by LC-MS/MS as TDVLGLPAK (912.5 Da) and DPNAALPAGPR (1,148.6 Da), and their IC₅₀ values were 153.4 and 95.7 μM, respectively. The results of this study therefore indicate that leukocyte extract of C. siamensis contains peptides with antioxidant activity which could be used as a novel antioxidant. |
| Databáze: | OpenAIRE |
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