Tanscriptomic Study of the Soybean-Fusarium virguliforme Interaction Revealed a Novel Ankyrin-Repeat Containing Defense Gene, Expression of Whose during Infection Led to Enhanced Resistance to the Fungal Pathogen in Transgenic Soybean Plants
| Autor: | Mohammad Samir Farooqi, Subodh K. Srivastava, Madan K. Bhattacharyya, Sekhar Kambakam, Binod Bihari Sahu, Sivakumar Swaminathan, Micheline N. Ngaki, Bing Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hydrolases Agricultural Biotechnology Cell Membranes lcsh:Medicine Gene Expression Artificial Gene Amplification and Extension Genetically modified crops Plant Science 01 natural sciences Plant Roots Genetically Modified Plants Biochemistry Polymerase Chain Reaction Fusarium Gene expression Plant defense against herbivory lcsh:Science Pathogen Disease Resistance Plant Proteins Genetics Multidisciplinary Plant Biochemistry Genetically Modified Organisms Agriculture Plants Plants Genetically Modified Ankyrin Repeat Enzymes Plant Physiology Host-Pathogen Interactions Cellular Structures and Organelles Genetic Engineering Research Article Biotechnology Crops Plant disease resistance Biology Research and Analysis Methods 03 medical and health sciences Plant Defenses Molecular Biology Techniques Gene Molecular Biology Plant Diseases Gene Expression Profiling lcsh:R Organisms Reproducibility of Results Biology and Life Sciences Membrane Proteins Proteins Cell Biology Reverse Transcriptase-Polymerase Chain Reaction Sudden infant death syndrome Gene expression profiling 030104 developmental biology Enzymology lcsh:Q Plant Biotechnology Soybeans Soybean 010606 plant biology & botany Crop Science |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 10, p e0163106 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Fusarium virguliforme causes the serious disease sudden death syndrome (SDS) in soybean. Host resistance to this pathogen is partial and is encoded by a large number of quantitative trait loci, each conditioning small effects. Breeding SDS resistance is therefore challenging and identification of single-gene encoded novel resistance mechanisms is becoming a priority to fight this devastating this fungal pathogen. In this transcriptomic study we identified a few putative soybean defense genes, expression of which is suppressed during F. virguliforme infection. The F. virguliforme infection-suppressed genes were broadly classified into four major classes. The steady state transcript levels of many of these genes were suppressed to undetectable levels immediately following F. virguliforme infection. One of these classes contains two novel genes encoding ankyrin repeat-containing proteins. Expression of one of these genes, GmARP1, during F. virguliforme infection enhances SDS resistance among the transgenic soybean plants. Our data suggest that GmARP1 is a novel defense gene and the pathogen presumably suppress its expression to establish compatible interaction. |
| Databáze: | OpenAIRE |
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