An Anti-Human CD13 Monoclonal Antibody That Suppresses the Suppressive Function of Treg Cells
| Autor: | Zhi-Jun Ge, Yan Ge, Peng Yang, Yun Zhang, Songguang Ju, Zhihong Cao, Yanpin Zhao, Haorong Wu, Songwen Ju, Wei Chen, Kai Zhang, Yong-Fei Tan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.drug_class
Immunology CD13 Antigens Biology Antibodies Monoclonal Humanized Monoclonal antibody T-Lymphocytes Regulatory B7-H1 Antigen Flow cytometry Mice Transforming Growth Factor beta Cell Line Tumor medicine Animals Humans Immunology and Allergy IL-2 receptor Mice Inbred BALB C Metalloproteinase medicine.diagnostic_test Antibodies Monoclonal Flow Cytometry Molecular biology Interleukin-10 Gene Expression Regulation Cell culture Monoclonal Cancer cell biology.protein Antibody |
| Zdroj: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32:16-20 |
| ISSN: | 2167-9436 |
| DOI: | 10.1089/mab.2012.0078 |
| Popis: | CD13 (CD13/aminopeptidase N, APN, or CD13/APN) is a widely expressed type II membrane-bound metalloprotease. It is often overexpressed on cancer cells and expressed on CD4(+)CD25(hi) Treg cell subpopulation with higher suppressive ability. It has been determined to be a promising target in cancer diagnosis and therapy. In this study, a functional anti-human CD13 monoclonal antibody, MAb 9E4, was obtained and the specificity of this MAb was verified by flow cytometry. This MAb effectively recognized the CD13 molecule expressed on a series of malignant cell lines. Furthermore, we demonstrated that MAb 9E4 suppresses the suppressive function of Treg cells. This functional anti-human CD13 MAb provides a valuable tool for further study targeting the CD13 positive Treg cells. |
| Databáze: | OpenAIRE |
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