Erratum to: Transcriptome and proteome mapping in the sheep atria reveal molecular features of atrial fibrillation progression
| Autor: | Alba Alvarez-Franco, Raquel Rouco, Rafael J Ramirez, Guadalupe Guerrero-Serna, Maria Tiana, Sara Cogliati, Kuljeet Kaur, Mohammed Saeed, Ricardo Magni, Jose Antonio Enriquez, Fatima Sanchez-Cabo, José Jalife, Miguel Manzanares |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics Time Factors Errata Proteome Physiology Gene Expression Profiling Action Potentials Disease Models Animal Heart Rate Physiology (medical) Atrial Fibrillation Disease Progression Animals AcademicSubjects/MED00200 Heart Atria Cardiology and Cardiovascular Medicine Transcriptome Sheep Domestic |
| Zdroj: | Cardiovascular Research |
| ISSN: | 1755-3245 0008-6363 |
| Popis: | Atrial fibrillation (AF) is a progressive cardiac arrhythmia that increases the risk of hospitalization and adverse cardiovascular events. There is a clear demand for more inclusive and large-scale approaches to understand the molecular drivers responsible for AF, as well as the fundamental mechanisms governing the transition from paroxysmal to persistent and permanent forms. In this study, we aimed to create a molecular map of AF and find the distinct molecular programmes underlying cell type-specific atrial remodelling and AF progression.We used a sheep model of long-standing, tachypacing-induced AF, sampled right and left atrial tissue, and isolated cardiomyocytes (CMs) from control, intermediate (transition), and late time points during AF progression, and performed transcriptomic and proteome profiling. We have merged all these layers of information into a meaningful three-component space in which we explored the genes and proteins detected and their common patterns of expression. Our data-driven analysis points at extracellular matrix remodelling, inflammation, ion channel, myofibril structure, mitochondrial complexes, chromatin remodelling, and genes related to neural function, as well as critical regulators of cell proliferation as hallmarks of AF progression. Most important, we prove that these changes occur at early transitional stages of the disease, but not at later stages, and that the left atrium undergoes significantly more profound changes than the right atrium in its expression programme. The pattern of dynamic changes in gene and protein expression replicate the electrical and structural remodelling demonstrated previously in the sheep and in humans, and uncover novel mechanisms potentially relevant for disease treatment.Transcriptomic and proteomic analysis of AF progression in a large animal model shows that significant changes occur at early stages, and that among others involve previously undescribed increase in mitochondria, changes to the chromatin of atrial CMs, and genes related to neural function and cell proliferation. |
| Databáze: | OpenAIRE |
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