Cochlear Repair by Transplantation of Human Cord Blood CD133+ Cells to Nod-Scid Mice Made Deaf with Kanamycin and Noise
| Autor: | Serena Urbani, Stavros Hatzopoulos, Andrea Ciorba, Richard Poulsom, Valeria Franceschini, Sara Magosso, Michele Cilli, Roberto P. Revoltella, Rosemary Jeffery, Guiscardo Lorito, Emanuela Ognio, A. Martini, Alfredo Rosellini, Riccardo Saccardi, Pietro Giordano, Lucia Bertolaso, Sandra Papini, Edi Simoni, Monica Michelini |
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| Přispěvatelé: | REVOLTELLA R.P., PAPINI S., ROSELLINI A., MICHELINI M., FRANCESCHINI V., CIORBA A., BERTOLASO L., MAGOSSO S., HATZOPOULOS S., LORITO G., GIORDANO P., SIMONI E., MARTINI A., OGNIO E., CILLI M., SACCARDI R., URBANI S., JEFFERY R., POULSOM R. |
| Rok vydání: | 2008 |
| Předmět: |
Transplantation
Heterologous Biomedical Engineering lcsh:Medicine Mice SCID Nod Deafness Scid mice Chimerism Mice Antigens CD Kanamycin Mice Inbred NOD otorhinolaryngologic diseases Animals Humans Medicine AC133 Antigen In Situ Hybridization Fluorescence Aged Glycoproteins Transplantation Cochlea repair Organ of corti Stem cell business.industry lcsh:R Hematopoietic Stem Cell Transplantation Cell Biology Fetal Blood Anti-Bacterial Agents Cochlea Haematopoiesis medicine.anatomical_structure Organ of Corti Cord blood Immunology Female sense organs Noise Peptides business medicine.drug |
| Zdroj: | Cell Transplantation, Vol 17 (2008) |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.3727/096368908786092685 |
| Popis: | We investigated the fate of human cord blood CD133+ hematopoietic stem cells (HSC) transplanted intravenously (IV) into irradiated nodscid mice previously made deaf by ototoxic treatment with kanamycin and/or intense noise, to verify whether HSC engraft the cochlea and contribute to inner ear restoration, in vivo. We tested the presence of HLA.DQα1 by PCR, used for traceability of engrafted cells, finding evidence that HSC migrated to various host tissues, including the organ of Corti (OC). By histology, antibody and lectin-staining analysis, we confirmed that HSC IV transplantation in mice previously damaged by ototoxic agents correlated with the repair process and stimulation ex novo of morphological recovery in the inner ear, while the cochlea of control oto-injured, nontransplanted mice remained seriously damaged. Dual color FISH analysis also provided evidence of positive engraftment in the inner ear and in various mouse tissues, also revealing small numbers of heterokaryons, probably derived from fusion of donor with endogenous cells, for up to 2 months following transplantation. These observations offer the first evidence that transplanted human HSC migrating to the inner ear of oto-injured mice may provide conditions for the resumption of deafened cochlea, emerging as a potential strategy for inner ear rehabilitation. |
| Databáze: | OpenAIRE |
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