Studies in the diabetic Chinese hamster: Light microscopy and autoradiography of pancreatic islets
| Autor: | W. E. Dulin, G. C. Gerritsen, A. A. Like, P. Gaudreau |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Remission Spontaneous Biology Tritium Chinese hamster Islets of Langerhans Cricetinae Internal medicine Diabetes mellitus Diabetes Mellitus Internal Medicine Hyperinsulinemia medicine Animals Insulin Glucocorticoids geography geography.geographical_feature_category Pancreatic islets Hyperplasia medicine.disease Islet biology.organism_classification Disease Models Animal Endocrinology medicine.anatomical_structure Hyperglycemia Autoradiography Beta cell |
| Zdroj: | Diabetologia. 10:501-508 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf01221980 |
| Popis: | Light microscopic and autoradiographic studies were performed in normal genetic nondiabetic and diabetic Chinese hamsters after the administration of thymidine-3H and correlated with levels of blood glucose (BG) and plasma insulin (IRI). Pancreatic islets of normal hamsters contained well granulated beta cells; rare islet cells incorporated thymidine-3H and the IRI/BG ratio (I/G) was =1.91. Recent onset diabetics revealed hyperglycemia and hyperinsulinemia (I/G = 1.97), beta cell degranulation and increased islet cell labelling. With progression of diabetes, I/G ratios decreased (Non-ketotic animals: 1.08, Ketotic hamsters: 0.17), beta cell numbers declined and islet labelling was infrequent. Hamsters with spontaneous remission from diabetes showed normoglycemia, hyperinsulinemia (I/G = 2.84) and beta cell hyperplasia. Glucocorticoid administration to normal hamsters induced marked BG elevations, beta cell hyperplasia and increased thymidine-3H incorporation. The absence of increased beta cell labelling among most diabetics treated with glucocorticoids may be a manifestation of a genetically defined defect of beta cell replication that is in part responsible for the declining beta cell mass and insulin synthetic capacity of the diabetic Chinese hamsters. |
| Databáze: | OpenAIRE |
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