Calcium-Dependent Protein Kinase CPK6 Positively Functions in Induction by Yeast Elicitor of Stomatal Closure and Inhibition by Yeast Elicitor of Light-Induced Stomatal Opening in Arabidopsis
| Autor: | Yoshimasa Nakamura, Yoshiyuki Murata, Izumi C. Mori, Shintaro Munemasa, Wenxiu Ye, Daichi Muroyama |
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| Rok vydání: | 2013 |
| Předmět: |
Light
Physiology Research Articles - Focus Issue Arabidopsis Saccharomyces cerevisiae Plant Science Biology chemistry.chemical_compound Guard cell Botany Genetics Arabidopsis thaliana Abscisic acid Methyl jasmonate Arabidopsis Proteins Protoplasts food and beverages Hydrogen Peroxide Biotic stress biology.organism_classification Apoplast Elicitor Cell biology chemistry Calcium-Calmodulin-Dependent Protein Kinases Mutation Plant Stomata Ion Channel Gating |
| Zdroj: | Plant Physiology. 163:591-599 |
| ISSN: | 1532-2548 |
| Popis: | Yeast elicitor (YEL) induces stomatal closure that is mediated by a Ca2+-dependent signaling pathway. A Ca2+-dependent protein kinase, CPK6, positively regulates activation of ion channels in abscisic acid and methyl jasmonate signaling, leading to stomatal closure in Arabidopsis (Arabidopsis thaliana). YEL also inhibits light-induced stomatal opening. However, it remains unknown whether CPK6 is involved in induction by YEL of stomatal closure or in inhibition by YEL of light-induced stomatal opening. In this study, we investigated the roles of CPK6 in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis. Disruption of CPK6 gene impaired induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening. Activation by YEL of nonselective Ca2+-permeable cation channels was impaired in cpk6-2 guard cells, and transient elevations elicited by YEL in cytosolic-free Ca2+ concentration were suppressed in cpk6-2 and cpk6-1 guard cells. YEL activated slow anion channels in wild-type guard cells but not in cpk6-2 or cpk6-1 and inhibited inward-rectifying K+ channels in wild-type guard cells but not in cpk6-2 or cpk6-1. The cpk6-2 and cpk6-1 mutations inhibited YEL-induced hydrogen peroxide accumulation in guard cells and apoplast of rosette leaves but did not affect YEL-induced hydrogen peroxide production in the apoplast of rosette leaves. These results suggest that CPK6 positively functions in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis and is a convergent point of signaling pathways for stomatal closure in response to abiotic and biotic stress. |
| Databáze: | OpenAIRE |
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